A low molecular weight (9600) glycoprotein was isolated from monkey (Macaca arctoides) extra-parotid saliva by gel filtration on Sephadex G-200 and Sephadex G-50. Criteria of purity included immunodiffusion, immunoelectrophoresis and polyacrylamide gel electrophoresis. The molecule contained 76 per cent protein, 23.4 per cent carbohydrate and 0.6 per cent phosphorus. The glycoprotein was a potent inhibitor of calcium phosphate precipitation.
Bibliographical noteFunding Information:
AcknoMlc,dyerncnrs~ We would like to acknowledge the excellent technical assistance of Mr. John P. Shomers and Mr. Gary E. Sadowski. This study wa supported in part by the National Institute of Dental Research. NIH, Training Grant No. 5T32DE07034. Research Grants No. IR23DE04518. No. SPOlDE03841 and N0. 5507RRO5330.