Isolation of a cdna for the catalytic subunit of rat liver glucose-6-phosphatase: Regulation of gene expression in fao hepatoma cells by insulin, dexamethasone and cAMP

Alex J. Lange, Doriane Argaud, M. Raafat Elmaghrabi, Wansong Pan, Subir R. Maitra, Simon J. Pilkis

Research output: Contribution to journalArticlepeer-review

116 Scopus citations

Abstract

cDNA clones coding for the catalytic subunit of rat liver glucose-6-phosphatase (EC 3.1.3.9) were isolated from a rat liver cDNA library in lambda gt11 phage. The sequence of the cDNA and the amino acid sequence derived from it were greater than 90% identical to the corresponding sequences for the mouse and human forms of liver glucose-6-phosphatase. Northern blot analysis of RNA from FAO hepatoma cells revealed that dexamethasone induced the glucose-6-phosphatase mRNA while insulin suppressed its expression. When both hormones were added together insulin completely suppressed the effect of glucocorticoid. cAMP addition alone decreased the abundance of glucose-6-phosphatase mRNA. The results demonstrate multihormonal regulation of gene expression of hepatic glucose-6-phosphatase and support a dominant role for insulin.

Original languageEnglish (US)
Pages (from-to)302-309
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume201
Issue number1
DOIs
StatePublished - May 30 1994

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