Isolation of 11 new polymorphic microsatellites from CA enriched Turkey genomic libraries (Short communication)

Edit Korom; Katalin Bakos; Gyula Veress; Orsolya Pinke; Kent Reed; László Varga; Balázs Kovács

doi:10.5194/aab-53-618-2010

Isolation of 11 new polymorphic microsatellites from CA enriched Turkey genomic libraries (Short communication)

Edit Korom, Katalin Bakos, Gyula Veress, Orsolya Pinke, Kent Reed, László Varga, Balázs Kovács

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Microsatellite loci from the ancient Hungarian variety of the Broad Breasted Bronze Turkey (Meleagris gallopavo) were isolated. CA-repeat enriched libraries were constructed from DNA of randomly collected samples. Libraries were screened for repeat-containing clones by PIMA (PCR Isolation of Microsatellite Arrays) and the DNA-sequence of 167 positive clones was determined. A total of 136 microsatellite repeat-containing sequences were found, 59 sequences were unique. Comparing these with the genomic databases, we found 7 previously annotated microsatellite sequences. The newly isolated 52 microsatellites were tested on the mapping population of the University of Minnesota, and the map position of 11 microsatellites was determined.

Original language	English (US)
Pages (from-to)	618-622
Number of pages	5
Journal	Archives Animal Breeding
Volume	53
Issue number	5
DOIs	https://doi.org/10.5194/aab-53-618-2010
State	Published - Oct 10 2010

Bibliographical note

Publisher Copyright:
© 2010 Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany.

Keywords

CA-enriched
genomic library
magnetic beads
microsatellite isolation
PIMA
Turkey (Meleagris gallopavo)

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10.5194/aab-53-618-2010

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title = "Isolation of 11 new polymorphic microsatellites from CA enriched Turkey genomic libraries (Short communication)",

abstract = "Microsatellite loci from the ancient Hungarian variety of the Broad Breasted Bronze Turkey (Meleagris gallopavo) were isolated. CA-repeat enriched libraries were constructed from DNA of randomly collected samples. Libraries were screened for repeat-containing clones by PIMA (PCR Isolation of Microsatellite Arrays) and the DNA-sequence of 167 positive clones was determined. A total of 136 microsatellite repeat-containing sequences were found, 59 sequences were unique. Comparing these with the genomic databases, we found 7 previously annotated microsatellite sequences. The newly isolated 52 microsatellites were tested on the mapping population of the University of Minnesota, and the map position of 11 microsatellites was determined.",

keywords = "CA-enriched, genomic library, magnetic beads, microsatellite isolation, PIMA, Turkey (Meleagris gallopavo)",

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note = "Publisher Copyright: {\textcopyright} 2010 Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany.",

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T1 - Isolation of 11 new polymorphic microsatellites from CA enriched Turkey genomic libraries (Short communication)

AU - Korom, Edit

AU - Bakos, Katalin

AU - Veress, Gyula

AU - Pinke, Orsolya

AU - Reed, Kent

AU - Varga, László

AU - Kovács, Balázs

PY - 2010/10/10

Y1 - 2010/10/10

N2 - Microsatellite loci from the ancient Hungarian variety of the Broad Breasted Bronze Turkey (Meleagris gallopavo) were isolated. CA-repeat enriched libraries were constructed from DNA of randomly collected samples. Libraries were screened for repeat-containing clones by PIMA (PCR Isolation of Microsatellite Arrays) and the DNA-sequence of 167 positive clones was determined. A total of 136 microsatellite repeat-containing sequences were found, 59 sequences were unique. Comparing these with the genomic databases, we found 7 previously annotated microsatellite sequences. The newly isolated 52 microsatellites were tested on the mapping population of the University of Minnesota, and the map position of 11 microsatellites was determined.

AB - Microsatellite loci from the ancient Hungarian variety of the Broad Breasted Bronze Turkey (Meleagris gallopavo) were isolated. CA-repeat enriched libraries were constructed from DNA of randomly collected samples. Libraries were screened for repeat-containing clones by PIMA (PCR Isolation of Microsatellite Arrays) and the DNA-sequence of 167 positive clones was determined. A total of 136 microsatellite repeat-containing sequences were found, 59 sequences were unique. Comparing these with the genomic databases, we found 7 previously annotated microsatellite sequences. The newly isolated 52 microsatellites were tested on the mapping population of the University of Minnesota, and the map position of 11 microsatellites was determined.

KW - CA-enriched

KW - genomic library

KW - magnetic beads

KW - microsatellite isolation

KW - PIMA

KW - Turkey (Meleagris gallopavo)

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Isolation of 11 new polymorphic microsatellites from CA enriched Turkey genomic libraries (Short communication)

Abstract

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