Isolation of 11 new polymorphic microsatellites from CA enriched Turkey genomic libraries (Short communication)

Edit Korom, Katalin Bakos, Gyula Veress, Orsolya Pinke, Kent Reed, László Varga, Balázs Kovács

Research output: Contribution to journalArticle

Abstract

Microsatellite loci from the ancient Hungarian variety of the Broad Breasted Bronze Turkey (Meleagris gallopavo) were isolated. CA-repeat enriched libraries were constructed from DNA of randomly collected samples. Libraries were screened for repeat-containing clones by PIMA (PCR Isolation of Microsatellite Arrays) and the DNA-sequence of 167 positive clones was determined. A total of 136 microsatellite repeat-containing sequences were found, 59 sequences were unique. Comparing these with the genomic databases, we found 7 previously annotated microsatellite sequences. The newly isolated 52 microsatellites were tested on the mapping population of the University of Minnesota, and the map position of 11 microsatellites was determined.

Original languageEnglish (US)
Pages (from-to)618-622
Number of pages5
JournalArchives Animal Breeding
Volume53
Issue number5
DOIs
StatePublished - Oct 10 2010

Keywords

  • CA-enriched
  • genomic library
  • magnetic beads
  • microsatellite isolation
  • PIMA
  • Turkey (Meleagris gallopavo)

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