Isolation, characterization, and developmental expression of pig intestinal fatty acid-binding proteins

Gregory A. Reinhart, Frank A. Simmen, Donald C. Mahan, Rosalia C M Simmen, Michael E. White

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The goal of this study was to characterize and quantify intestinal fatty acid-binding proteins of the pig. Small intestinal mucosa from 13-19 kg pigs was homogenized and centrifuged to obtain cytosol. Isolation of fatty acid-binding proteins from delipidated cytosol was achieved using molecular sieve, oleic acid affinity, and ion exchange chromatography. Fatty acid-binding protein isolation was monitored using a fatty-acid binding assay in conjunction with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. Antisera to rat liver-fatty acid-binding protein cross reacted with an isolated intestinal fatty acid-binding protein of Mr = 13,000, whereas antisera to rat intestine-fatty acid-binding protein was not cross reactive with isolated pig intestinal proteins. These experiments identify a pig intestinal fatty acid-binding protein that exhibits strong immunochemical similarity to rat liver-fatty acid-binding protein. Cytosol prepared from intestinal mucosa of pigs at -4, 2, 4, 7, 15, 22, 28, and 35 d of age was assayed for fatty acid-binding protein activity. Preweaning fatty acid-binding protein activity in cytosol was maximal at 7 days of age when expressed as total jejunal fatty acid binding per kilogram bodyweight, intestinal or mucosal weight or milligram total protein. After weaning (21 d), fatty acid-binding protein activities declined to 28 days, but increased again by 35 days. Total soluble fatty acid-binding protein activity in pig intestine is regulated during postnatal development and this may account in part for the altered intestinal absorption of lipids observed in young pigs at weaning.

Original languageEnglish (US)
Pages (from-to)592-598
Number of pages7
JournalThe Journal of Nutritional Biochemistry
Volume1
Issue number11
DOIs
StatePublished - Nov 1990

Keywords

  • fatty acid binding protein
  • lipid metabolism
  • ontogeny
  • pig
  • small intestine

Fingerprint Dive into the research topics of 'Isolation, characterization, and developmental expression of pig intestinal fatty acid-binding proteins'. Together they form a unique fingerprint.

Cite this