Isolation and sequence of a gene encoding a methionine-rich 10-kDa zein protein from maize

Julie Anderson Kirihara, Jean Bernhard Petri, Joachim Messing

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

We have isolated the gene encoding a methionine-rich 10-kDa zein protein from a λEMBL3 maize genomic 'mini' library of the inbred line BSSS-53 and determined its nucleotide sequence. The sequence matches perfectly with a cDNA clone from the inbred line W22 (which has the same restriction fragment length polymorphism as many inbred Unes tested) indicating that we have isolated a functional storage protein gene that is very conserved in maize. This comparison also excludes any splicing of any precursor mRNA and therefore any presence of introns. A number of potential regulatory sequences have been located in the flanking regions. The 10-kDa-zein gene represents the last size class in the zein multigene family to be characterized. Its structure allows us now to re-examine the relationship of all the zein proteins and also to compare the structure of a new class of storage proteins that are rich in methionine, an essential amino acid in livestock fodder.

Original languageEnglish (US)
Pages (from-to)359-370
Number of pages12
JournalGene
Volume71
Issue number2
DOIs
StatePublished - Nov 30 1988

Keywords

  • Recombinant DNA
  • Zea mays
  • evolution of a new class of storage proteins
  • genomic mini-library
  • phage λ vectors
  • prolamines

Fingerprint Dive into the research topics of 'Isolation and sequence of a gene encoding a methionine-rich 10-kDa zein protein from maize'. Together they form a unique fingerprint.

Cite this