Isolation and partial characterization of the major amidelinked conjugate of indole-3-acetic acid from Phaseolus vulgaris L.1

Krystyna Bialek, Jerry D. Cohen

Research output: Contribution to journalArticle

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Abstract

A major indole-3-acetic acid conjugate from Phaseolus vulgaris seed has been isolated and partially characterized. It is a 3 kilodalton peptide with apparently 2 indole-3-acetyl moieties in amide linkage per peptide. The indole-3-acetic acid component was identified by gas chromatography-mass spectrometry and the peptide characterized by polyacrylamide gel electrophoresis, by amino acid analysis using dabsyl derivatives and by its Fourier transform-infrared spectrum. This is the first higher molecular weight amide-linked indole-3-acetic acid conjugate to be characterized from higher plants.

Original languageEnglish (US)
Pages (from-to)99-104
Number of pages6
JournalPlant physiology
Volume80
Issue number1
DOIs
StatePublished - Dec 1 1986

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