Isolation and molecular characterization of a novel picornavirus from baitfish in the USA

Nicholas B D Phelps, Sunil K. Mor, Anibal G. Armien, William Batts, Andrew E. Goodwin, Lacey Hopper, Rebekah McCann, Terry Fei Fan Ng, Corey Puzach, Thomas B. Waltzek, Eric Delwart, James Winton, Sagar M. Goyal

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

During both regulatory and routine surveillance sampling of baitfish from the states of Illinois, Minnesota, Montana, and Wisconsin, USA, isolates (n = 20) of a previously unknown picornavirus were obtained from kidney/spleen or entire viscera of fathead minnows (Pimephales promelas) and brassy minnows (Hybognathus hankinsoni). Following the appearance of a diffuse cytopathic effect, examination of cell culture supernatant by negative contrast electron microscopy revealed the presence of small, round virus particles (∼30-32 nm), with picornavirus-like morphology. Amplification and sequence analysis of viral RNA identified the agent as a novel member of the Picornaviridae family, tentatively named fathead minnow picornavirus (FHMPV). The full FHMPV genome consisted of 7834 nucleotides. Phylogenetic analysis based on 491 amino acid residues of the 3D gene showed 98.6% to 100% identity among the 20 isolates of FHMPV compared in this study while only 49.5% identity with its nearest neighbor, the bluegill picornavirus (BGPV) isolated from bluegill (Lepomis macrochirus). Based on complete polyprotein analysis, the FHMPV shared 58% (P1), 33% (P2) and 43% (P3) amino acid identities with BGPV and shared less than 40% amino acid identity with all other picornaviruses. Hence, we propose the creation of a new genus (Piscevirus) within the Picornaviridae family. The impact of FHMPV on the health of fish populations is unknown at present.

Original languageEnglish (US)
Article numbere87593
JournalPloS one
Volume9
Issue number2
DOIs
StatePublished - Feb 21 2014

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baitfish
Picornaviridae
Pimephales promelas
Cyprinidae
Amino Acids
Genes
Polyproteins
Lepomis macrochirus
Viral RNA
Viruses
Cell culture
Perciformes
Fish
Electron microscopy
Amplification
Nucleotides
Health
Sampling
amino acids
fish health

Cite this

Isolation and molecular characterization of a novel picornavirus from baitfish in the USA. / Phelps, Nicholas B D; Mor, Sunil K.; Armien, Anibal G.; Batts, William; Goodwin, Andrew E.; Hopper, Lacey; McCann, Rebekah; Ng, Terry Fei Fan; Puzach, Corey; Waltzek, Thomas B.; Delwart, Eric; Winton, James; Goyal, Sagar M.

In: PloS one, Vol. 9, No. 2, e87593, 21.02.2014.

Research output: Contribution to journalArticle

Phelps, NBD, Mor, SK, Armien, AG, Batts, W, Goodwin, AE, Hopper, L, McCann, R, Ng, TFF, Puzach, C, Waltzek, TB, Delwart, E, Winton, J & Goyal, SM 2014, 'Isolation and molecular characterization of a novel picornavirus from baitfish in the USA', PloS one, vol. 9, no. 2, e87593. https://doi.org/10.1371/journal.pone.0087593
Phelps, Nicholas B D ; Mor, Sunil K. ; Armien, Anibal G. ; Batts, William ; Goodwin, Andrew E. ; Hopper, Lacey ; McCann, Rebekah ; Ng, Terry Fei Fan ; Puzach, Corey ; Waltzek, Thomas B. ; Delwart, Eric ; Winton, James ; Goyal, Sagar M. / Isolation and molecular characterization of a novel picornavirus from baitfish in the USA. In: PloS one. 2014 ; Vol. 9, No. 2.
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