Isolation and characterization of vitamin K dependent region of bovine blood clotting factor X

J. B. Howard, Gary L Nelsestuen

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

A 39 residue peptide from the tryptic digestion of bovine blood clotting factor X has been isolated by specific adsorption on barium citrate. The amino and carboxyl terminal sequences of the peptide were determined and compared to the vitamin K dependent Ca2+ binding region from bovine prothrombin. The factor X peptide was found to contain γ carboxyglutamic acid residues, and the results of independent analysis are consistent with all 14 glutamic acid residues as γ carboxyglutamic acid. The similarity of the factor X peptide to the prothrombin peptide supports the hypothesis that the vitamin K dependent blood clotting proteins are descended from a common ancestral gene.

Original languageEnglish (US)
Pages (from-to)1281-1285
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number4
DOIs
StatePublished - 1975

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