Isolation and characterization of estuarine dissolved organic matter

Comparison of ultrafiltration and C18 solid-phase extraction techniques

Jean Paul Simjouw, Elizabeth C. Minor, Kenneth Mopper

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Characterization of dissolved organic matter (DOM) from aquatic environments has always been constrained by the ability to obtain a representative fraction of the DOM pool for analysis. Ultrafiltration or extraction, commonly using XAD or C18 sorbents, is therefore generally used to concentrate and desalt DOM samples for further analyses. In this study, we compared ultrafiltration and C18 solid-phase extraction disks (SPE) as DOM isolation methods for estuarine samples. We also evaluated the use of the C18 SPE disks to isolate low-molecular-weight DOM (LMW-DOM) in the filtrate from ultrafiltration. The isolates from both methods and the LMW-DOM C18-extracts were characterized using Fourier transform infrared spectroscopy (FTIR) and direct temperature-resolved mass spectrometry (DT-MS). Based on mass balance and blank measurements, we found that the C18 SPE disks can be used to isolate bulk DOM and LMW-DOM from estuarine samples. FTIR and DT-MS analysis show that C18-extracted DOM and ultrafiltered high-molecular-weight DOM (HMW-DOM) differ markedly in chemical composition. The HMW-DOM is enriched in (degraded) polysaccharides along with aminosugars when compared with the C 18-extracted DOM. The C18-extracted DOM appears enriched in aromatic compounds, probably from lignin and/or aromatic amino acids in proteins. C18 SPE of LMW-DOM samples from ultrafiltration increases the recovery of DOM from the total sample up to about 70%, compared to around 50% using ultrafiltration alone. Thus, a majority of the DOM can be isolated from estuarine samples by a combination of these techniques.

Original languageEnglish (US)
Pages (from-to)219-235
Number of pages17
JournalMarine Chemistry
Volume96
Issue number3-4
DOIs
StatePublished - Sep 1 2005

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Ultrafiltration
ultrafiltration
dissolved organic matter
Biological materials
2,5-Dimethoxy-4-Methylamphetamine
Molecular weight
FTIR spectroscopy
Mass spectrometry
comparison
mass spectrometry
Aromatic Amino Acids
Aromatic compounds
Lignin
Sorbents
polysaccharide
aquatic environment
lignin
Polysaccharides
mass balance
amino acid

Keywords

  • C extraction
  • Characterization
  • Dissolved organic matter
  • FTIR spectroscopy
  • Mass spectrometry
  • Ultrafiltration

Cite this

Isolation and characterization of estuarine dissolved organic matter : Comparison of ultrafiltration and C18 solid-phase extraction techniques. / Simjouw, Jean Paul; Minor, Elizabeth C.; Mopper, Kenneth.

In: Marine Chemistry, Vol. 96, No. 3-4, 01.09.2005, p. 219-235.

Research output: Contribution to journalArticle

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