Isolation and characterization of cycloheximide-resistant mosquito cell clones

Ann Marie Fallon, Victor Stollar

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Following treatment of cultured mosquito cells (Aedes albopictus line of Singh) with the chemical mutagen ethyl methanesulfonate, we were able to isolate three cycloheximide-resistant clones. On the basis of growth kinetics, plating efficiency, and protein synthesis, these clones are 10- to 30-fold more resistant to cycloheximide than the parent cells. Cell-free lysates made from these cells retain 30-65% of their endogenous protein synthesizing ability in the presence of cycloheximide concentrations as high as 300 μg/ml. Protein synthesis in lysates from the parental cells, however, is reduced to about 10% of the control value (i.e., in the absence of drug) at 14 μg of cycloheximide/ml and was completely abolished at 75 μg/ml. These results indicate that cycloheximide resistance in these cells is likely due to an alteration in the protein synthetic machinery. This is the first description of cycloheximide-resistant insect cells, and the best example of cycloheximide resistance in cells originating from a higher eukaryotic organism.

Original languageEnglish (US)
Pages (from-to)201-212
Number of pages12
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume96
Issue number2-3
DOIs
StatePublished - Oct 1982

Bibliographical note

Funding Information:
This investigation was supported by Grant AI-11290 from the National Institute of Allergy and Infectious Diseases. A.M.F. was supported by an N.I.H. postdoctoral fellowship. We thank Lily Tao for technical assistance.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

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