Isolation and characterization of complementation products of Escherichia coli alkaline phosphatase

D. P. Fan, M. J. Schlesinger, A. Torriani, K. J. Barrett, C. Levinthal

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11 Scopus citations

Abstract

Hybrid dimers of the Escherichia coli alkaline phosphatase were formed in vitro with one subunit each from various mutants. When one of the subunit species was labeled for density (D, 15N) and for radioactivity, it was possible to isolate the hybrids and study their properties directly. Such studies showed that some subunit species combine with greater affinity than others and that different hybrids can have different specific enzymic activities. The genetic map for the mutants studied is presented and its relationship to complementation is discussed.

Original languageEnglish (US)
Pages (from-to)32-48
Number of pages17
JournalJournal of Molecular Biology
Volume15
Issue number1
DOIs
StatePublished - 1966

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Copyright 2017 Elsevier B.V., All rights reserved.

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