Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci

Karen M. Léon-Kloosterziel, Marta Alvarez Gil, Gerda J. Ruijs, Steven E. Jacobsen, Neil E Olszewski, Steven H. Schwartz, Jan A.D. Zeevaart, Maarten Koornneef

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Abstract

Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA-deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA-deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.

Original languageEnglish (US)
Pages (from-to)655-661
Number of pages7
JournalPlant Journal
Volume10
Issue number4
DOIs
StatePublished - Oct 1 1996

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    Léon-Kloosterziel, K. M., Gil, M. A., Ruijs, G. J., Jacobsen, S. E., Olszewski, N. E., Schwartz, S. H., Zeevaart, J. A. D., & Koornneef, M. (1996). Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci. Plant Journal, 10(4), 655-661. https://doi.org/10.1046/j.1365-313X.1996.10040655.x