Isolation and characterization of a new transposable element in Chlamydomonas reinhardtii

Shao Chun Wang, Rogene A. Schnell, Paul A. Lefebvre

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

A new transposable element, Tcr3, was identified in the unicellular green alga Chlamydomonas reinhardtii. The Tcr3 element contained imperfect terminal inverted repeat sequences of 56 bp and created a 2 bp target site duplication upon insertion. Insertion of Tcr3 into the 3'-untranslated region of the NIT8 gene, which is essential for nitrate assimilation, prevented expression of the gene. Excision of the Tcr3 element correlated with reversion of the mutant phenotype and left behind a 3 bp footprint. Tcr3 was found in all Chlamydomonas isolates tested and should prove to be useful for transposon-tagging experiments in Chlamydomonas.

Original languageEnglish (US)
Pages (from-to)681-687
Number of pages7
JournalPlant molecular biology
Volume38
Issue number5
DOIs
StatePublished - Nov 1998

Bibliographical note

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

Keywords

  • Chlamydomonas reinhardtii
  • NIT8
  • Nitrate assimilation
  • Tcr3
  • Transposable element

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