Isolation and characterization of βact in gene of Carp (cyprinus carpio)

Zhanjiang Liu; Zuoyan Zhu; Kevin Roberg; Anthony Faras; Kevin Guise; Anne R. Kapuscinski; Perry B Hackett

doi:10.3109/10425179009016040

Isolation and characterization of βact in gene of Carp (cyprinus carpio)

Zhanjiang Liu
, Zuoyan Zhu
, Kevin Roberg
, Anthony Faras
, Kevin Guise
, Anne R. Kapuscinski
, Perry B Hackett

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

A βactin gene of carp (Cyprinus carpio) was isolated from a genomic EMBL3 library. The nucleotide sequence of the gene indicates six exons spanning 3.6 kb. Southern blot hybridization of restriction endonuclease digests of carp genomic DNA indicate that there are two copies of the βactin isotype and several other species of actin genes. The transcriptional start site is 85 bp and 24 bp downstream respectively from consensus CCAAT and TATA promoter elements. The organization of the carp βactin gene is identical to that of chicken, human, and rat genes in terms of size, exon/intron locations and junctions and in having a translationally silent first exon. The fish gene is 90% and 99% conserved at the nucleotide and amino acid levels, respectively, with land vertebrate βactin genes. Northern blot analysis of βactin gene expression indicated that the gene is highly expressed in brain, less so in muscle, and much less so in liver cells. The putative βactin proximal promoter of carp, identified by the conservation of known actin regulatory sequences, is transcriptionally active in both mammalian and piscine cells.

Original language	English (US)
Pages (from-to)	125-136
Number of pages	12
Journal	Mitochondrial DNA
Volume	1
Issue number	2
DOIs	https://doi.org/10.3109/10425179009016040
State	Published - 1990

Bibliographical note

Funding Information:
We thank Ling He, Yuefeng Xie, Guohua Li and Kesheng Xu (Institute of Hydrobiology, Wuhan, People’s Republic of China) for help in the preparation of the carp genomic library; Steve Hughes for providing his chicken 8-actin gene clone; Aris Moustakas and Boaz Moav for discussions and reading the manuscript; Peter Saurugger and Darrin Johnson (University of Minnesota Molecular Biology Computing Center) for help with the lntelligenetics Programs; Kris Kirkeby and Toots McTavish for preparing the figures; and Carol Heiser for typing the manuscript. This work was supported by grants from NIH and the Minnesota Sea Grant College Program, supported by the NOAA Office of Sea Grant, DOC to P.H. Z.L. was supported by a stipend from Minnesota Sea Grant; Z.Z. was supported by a University of Minnesota Hill Visiting Professorship. The sequence has been deposited in the GenBank database under the accession number M241134.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 15 Life on Land

Keywords

Evolutionary conservation
Genomic library
Mapping
Promoter
Tissue-specific expression

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10.3109/10425179009016040

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title = "Isolation and characterization of βact in gene of Carp (cyprinus carpio)",

abstract = "A βactin gene of carp (Cyprinus carpio) was isolated from a genomic EMBL3 library. The nucleotide sequence of the gene indicates six exons spanning 3.6 kb. Southern blot hybridization of restriction endonuclease digests of carp genomic DNA indicate that there are two copies of the βactin isotype and several other species of actin genes. The transcriptional start site is 85 bp and 24 bp downstream respectively from consensus CCAAT and TATA promoter elements. The organization of the carp βactin gene is identical to that of chicken, human, and rat genes in terms of size, exon/intron locations and junctions and in having a translationally silent first exon. The fish gene is 90\% and 99\% conserved at the nucleotide and amino acid levels, respectively, with land vertebrate βactin genes. Northern blot analysis of βactin gene expression indicated that the gene is highly expressed in brain, less so in muscle, and much less so in liver cells. The putative βactin proximal promoter of carp, identified by the conservation of known actin regulatory sequences, is transcriptionally active in both mammalian and piscine cells.",

keywords = "Evolutionary conservation, Genomic library, Mapping, Promoter, Tissue-specific expression",

author = "Zhanjiang Liu and Zuoyan Zhu and Kevin Roberg and Anthony Faras and Kevin Guise and Kapuscinski, \{Anne R.\} and Hackett, \{Perry B\}",

note = "Funding Information: We thank Ling He, Yuefeng Xie, Guohua Li and Kesheng Xu (Institute of Hydrobiology, Wuhan, People{\textquoteright}s Republic of China) for help in the preparation of the carp genomic library; Steve Hughes for providing his chicken 8-actin gene clone; Aris Moustakas and Boaz Moav for discussions and reading the manuscript; Peter Saurugger and Darrin Johnson (University of Minnesota Molecular Biology Computing Center) for help with the lntelligenetics Programs; Kris Kirkeby and Toots McTavish for preparing the figures; and Carol Heiser for typing the manuscript. This work was supported by grants from NIH and the Minnesota Sea Grant College Program, supported by the NOAA Office of Sea Grant, DOC to P.H. Z.L. was supported by a stipend from Minnesota Sea Grant; Z.Z. was supported by a University of Minnesota Hill Visiting Professorship. The sequence has been deposited in the GenBank database under the accession number M241134.",

year = "1990",

doi = "10.3109/10425179009016040",

language = "English (US)",

volume = "1",

pages = "125--136",

journal = "Mitochondrial DNA",

issn = "1940-1744",

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T1 - Isolation and characterization of βact in gene of Carp (cyprinus carpio)

AU - Liu, Zhanjiang

AU - Zhu, Zuoyan

AU - Roberg, Kevin

AU - Faras, Anthony

AU - Guise, Kevin

AU - Kapuscinski, Anne R.

AU - Hackett, Perry B

N1 - Funding Information: We thank Ling He, Yuefeng Xie, Guohua Li and Kesheng Xu (Institute of Hydrobiology, Wuhan, People’s Republic of China) for help in the preparation of the carp genomic library; Steve Hughes for providing his chicken 8-actin gene clone; Aris Moustakas and Boaz Moav for discussions and reading the manuscript; Peter Saurugger and Darrin Johnson (University of Minnesota Molecular Biology Computing Center) for help with the lntelligenetics Programs; Kris Kirkeby and Toots McTavish for preparing the figures; and Carol Heiser for typing the manuscript. This work was supported by grants from NIH and the Minnesota Sea Grant College Program, supported by the NOAA Office of Sea Grant, DOC to P.H. Z.L. was supported by a stipend from Minnesota Sea Grant; Z.Z. was supported by a University of Minnesota Hill Visiting Professorship. The sequence has been deposited in the GenBank database under the accession number M241134.

PY - 1990

Y1 - 1990

N2 - A βactin gene of carp (Cyprinus carpio) was isolated from a genomic EMBL3 library. The nucleotide sequence of the gene indicates six exons spanning 3.6 kb. Southern blot hybridization of restriction endonuclease digests of carp genomic DNA indicate that there are two copies of the βactin isotype and several other species of actin genes. The transcriptional start site is 85 bp and 24 bp downstream respectively from consensus CCAAT and TATA promoter elements. The organization of the carp βactin gene is identical to that of chicken, human, and rat genes in terms of size, exon/intron locations and junctions and in having a translationally silent first exon. The fish gene is 90% and 99% conserved at the nucleotide and amino acid levels, respectively, with land vertebrate βactin genes. Northern blot analysis of βactin gene expression indicated that the gene is highly expressed in brain, less so in muscle, and much less so in liver cells. The putative βactin proximal promoter of carp, identified by the conservation of known actin regulatory sequences, is transcriptionally active in both mammalian and piscine cells.

AB - A βactin gene of carp (Cyprinus carpio) was isolated from a genomic EMBL3 library. The nucleotide sequence of the gene indicates six exons spanning 3.6 kb. Southern blot hybridization of restriction endonuclease digests of carp genomic DNA indicate that there are two copies of the βactin isotype and several other species of actin genes. The transcriptional start site is 85 bp and 24 bp downstream respectively from consensus CCAAT and TATA promoter elements. The organization of the carp βactin gene is identical to that of chicken, human, and rat genes in terms of size, exon/intron locations and junctions and in having a translationally silent first exon. The fish gene is 90% and 99% conserved at the nucleotide and amino acid levels, respectively, with land vertebrate βactin genes. Northern blot analysis of βactin gene expression indicated that the gene is highly expressed in brain, less so in muscle, and much less so in liver cells. The putative βactin proximal promoter of carp, identified by the conservation of known actin regulatory sequences, is transcriptionally active in both mammalian and piscine cells.

KW - Evolutionary conservation

KW - Genomic library

KW - Mapping

KW - Promoter

KW - Tissue-specific expression

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UR - https://www.scopus.com/pages/publications/0025524687#tab=citedBy

U2 - 10.3109/10425179009016040

DO - 10.3109/10425179009016040

M3 - Article

C2 - 2134183

AN - SCOPUS:0025524687

SN - 1940-1744

VL - 1

SP - 125

EP - 136

JO - Mitochondrial DNA

JF - Mitochondrial DNA

IS - 2

ER -

Isolation and characterization of βact in gene of Carp (cyprinus carpio)

Abstract

Bibliographical note

UN SDGs

Keywords

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