TY - JOUR
T1 - Isolation and analysis of small RNAs from virus-infected plants
AU - Curtin, Shaun J.
AU - Zsögön, Agustín
AU - Watson, John M.
AU - Waterhouse, Peter M.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - In this chapter, we detail some of the methods available to the researcher for isolating and analyzing virus-derived small RNAs (vsRNAs). These methods have been successfully used for four plant viruses: Cucumber mosaic virus (CMV), including the CMV Y-Satellite, Turnip mosaic virus (TuMV), Potato leaf roll virus (PLRV), and Tomato spotted wilt virus (TSWV) from inoculated Arabidopsis thaliana plants (Fusaro et al. EMBO Rep 7:1168-1175, 2006; Curtin et al. FEBS Lett 582:2753-2760, 2008). The protocols presented here can also be employed for the isolation of non-virus related small RNAs such as microRNAs (miRNAs) and hairpin RNA (hpRNA).
AB - In this chapter, we detail some of the methods available to the researcher for isolating and analyzing virus-derived small RNAs (vsRNAs). These methods have been successfully used for four plant viruses: Cucumber mosaic virus (CMV), including the CMV Y-Satellite, Turnip mosaic virus (TuMV), Potato leaf roll virus (PLRV), and Tomato spotted wilt virus (TSWV) from inoculated Arabidopsis thaliana plants (Fusaro et al. EMBO Rep 7:1168-1175, 2006; Curtin et al. FEBS Lett 582:2753-2760, 2008). The protocols presented here can also be employed for the isolation of non-virus related small RNAs such as microRNAs (miRNAs) and hairpin RNA (hpRNA).
KW - Total RNA extraction
KW - vsRNA
KW - vsRNA fractionation
KW - vsRNA northern blot analysis
UR - http://www.scopus.com/inward/record.url?scp=84934438705&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84934438705&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-882-5_12
DO - 10.1007/978-1-61779-882-5_12
M3 - Article
C2 - 22678580
AN - SCOPUS:84934438705
SN - 1064-3745
VL - 894
SP - 173
EP - 189
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -