Isolating Male Meiocytes from Maize and Wheat for “-Omics” Analyses

Stefanie Dukowic-Schulze, Nelson Garcia, Arun S.K. Shunmugam, Sateesh Kagale, Changbin Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Genome-wide gene expression studies have become a routine approach due to the advances in sequencing technologies, their ease of use, and increasing affordability. Simultaneous investigation of small RNA expression adds further valuable information but is not adopted as widely yet. Both RNA-seq and small RNA-seq benefit from the use of specific cell types. Here, we describe a protocol for the isolation of male meiotic cells from maize or wheat plants, along with the application of downstream RNA sequencing, extendable to other -omics approaches.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages237-258
Number of pages22
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2061
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Funding Information:
This work was supported by the National Science Foundation (IOS: 1025881, IOS-1546792), a grant-in-aid fund from the University of Minnesota, and Genome Canada funded Canadian Triticum Applied Genomics2 (CTAG2) initiative.

Keywords

  • Maize
  • Meiocytes
  • Meiosis
  • Omics
  • Plants
  • Sequencing
  • Wheat

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

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