Abstract
Genome-wide gene expression studies have become a routine approach due to the advances in sequencing technologies, their ease of use, and increasing affordability. Simultaneous investigation of small RNA expression adds further valuable information but is not adopted as widely yet. Both RNA-seq and small RNA-seq benefit from the use of specific cell types. Here, we describe a protocol for the isolation of male meiotic cells from maize or wheat plants, along with the application of downstream RNA sequencing, extendable to other -omics approaches.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 237-258 |
| Number of pages | 22 |
| Volume | 2061 |
| DOIs | |
| State | Published - 2020 |
Publication series
| Name | Methods in molecular biology (Clifton, N.J.) |
|---|---|
| Publisher | Humana Press |
| ISSN (Print) | 1064-3745 |
Bibliographical note
Funding Information:This work was supported by the National Science Foundation (IOS: 1025881, IOS-1546792), a grant-in-aid fund from the University of Minnesota, and Genome Canada funded Canadian Triticum Applied Genomics2 (CTAG2) initiative.
Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.
Keywords
- Maize
- Meiocytes
- Meiosis
- Omics
- Plants
- Sequencing
- Wheat
- Gene Expression Profiling
- Triticum/genetics
- Computational Biology/methods
- Genomics/methods
- Plant Development/genetics
- High-Throughput Nucleotide Sequencing
- Zea mays/genetics
- Gene Ontology
PubMed: MeSH publication types
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, Non-P.H.S.
- Journal Article