Isobutanol production in engineered Saccharomyces cerevisiae by overexpression of 2-ketoisovalerate decarboxylase and valine biosynthetic enzymes

Won Heong Lee, Seung Oh Seo, Yi Hyun Bae, Hong Nan, Yong Su Jin, Jin Ho Seo

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Engineering of Saccharomyces cerevisiae to produce advanced biofuels such as isobutanol has received much attention because this yeast has a natural capacity to produce higher alcohols. In this study, construction of isobutanol production systemswas attempted by overexpression of effective 2-keto acid decarboxylase (KDC) and combinatorial overexpression of valine biosynthetic enzymes in S. cerevisiae D452-2. Among the six putative KDC enzymes from various microorganisms, 2-ketoisovalerate decarboxylase (Kivd) from L. lactis subsp. lactis KACC 13877 was identified as the most suitableKDCfor isobutanol production in the yeast. Isobutanol production by the engineered S. cerevisiae was assessed in micro-aerobic batch fermentations using glucose as a sole carbon source. 93 mg/L isobutanol was produced in the Kivd overexpressing strain, which corresponds to a fourfold improvement as compared with the control strain. Isobutanol production was further enhanced to 151 mg/L by additional overexpression of acetolactate synthase (Ilv2p), acetohydroxyacid reductoisomerase (Ilv5p), and dihydroxyacid dehydratase (Ilv3p) in the cytosol.

Original languageEnglish (US)
Pages (from-to)1467-1475
Number of pages9
JournalBioprocess and Biosystems Engineering
Volume35
Issue number9
DOIs
StatePublished - Nov 1 2012

Keywords

  • 2-Ketoisovalerate decarboxylase
  • Engineered saccharomyces cerevisiae
  • Isobutanol
  • Valine biosynthetic enzymes

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