Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues

Carolina Tropini, Elizabeth A. Roth, Marija Zanic, Melissa K Gardner, Jonathon Howard

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Microtubules are dynamic polymers of GTP- and GDP-tubulin that undergo stochastic transitions between growing and shrinking phases. Rescues, the conversion from shrinking to growing, have recently been proposed to be to the result of regrowth at GTP-tubulin islands within the lattice of growing microtubules. By introducing mixed GTP/GDP/GMPCPP (GXP) regions within the lattice of dynamic microtubules, we reconstituted GXP islands in vitro (GMPCPP is the slowly hydrolyzable GTP analog guanosine-5′-[(α,β)-methyleno]triphosphate). We found that such islands could reproducibly induce rescues and that the probability of rescue correlated with both the size of the island and the percentage of GMPCPP-tubulin within the island. The islands slowed the depolymerization rate of shortening microtubules and promoted regrowth more readily than GMPCPP seeds. Together, these findings provide new mechanistic insights supporting the possibility that rescues could be triggered by enriched GTP-tubulin regions and present a new tool for studying such rescue events in vitro.

Original languageEnglish (US)
Article numbere30103
JournalPloS one
Volume7
Issue number1
DOIs
StatePublished - Jan 17 2012

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Guanosine Triphosphate
Islands
Microtubules
microtubules
Tubulin
tubulin
regrowth
Depolymerization
Guanosine
guanosine
depolymerization
Seed
Polymers
shortenings
Seeds
polymers
5'-guanylylmethylenebisphosphonate
seeds

Cite this

Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues. / Tropini, Carolina; Roth, Elizabeth A.; Zanic, Marija; Gardner, Melissa K; Howard, Jonathon.

In: PloS one, Vol. 7, No. 1, e30103, 17.01.2012.

Research output: Contribution to journalArticle

Tropini, Carolina ; Roth, Elizabeth A. ; Zanic, Marija ; Gardner, Melissa K ; Howard, Jonathon. / Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues. In: PloS one. 2012 ; Vol. 7, No. 1.
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