Ion transport and phosphoproteins of human red blood cells

J. D. Judah, K. Ahmed, A. E.M. McLean

Research output: Contribution to journalArticlepeer-review

103 Scopus citations

Abstract

1. 1. The influence of Na+ and K+ ions on the phosphate metabolism of the phosphoproteins of human red cells has been studied. 2. 2. With high intracellular Na+, but in the absence of external K+, the radioactivity of the phosphoproteins increases steadily over a period of at least 4 h. 3. 3. In the presence of external K+, or if external K+ is added during the incubation, the number of radioactive phosphate groups in the proteins declines and reaches a steady-state level at about 67% of that in the absence of external K+. 4. 4. Ouabain abolishes the effect of K+ ions, and also reduces the build up of radioactive phosphoprotein in the presence of intracellular Na+ ions. 5. 5. A protein phosphokinase has been demonstrated in purified red-cell membranes. It is activated by a number of monovalent cations. 6. 6. A phosphatase has been demonstrated to exist in these membranes. This enzyme splits p-nitrophenyl phosphate, is stimulated by K+ ions and inhibited by ouabain.

Original languageEnglish (US)
Pages (from-to)472-480
Number of pages9
JournalBBA - Biochimica et Biophysica Acta
Volume65
Issue number3
DOIs
StatePublished - Dec 17 1962

Bibliographical note

Funding Information:
This investigation was supported (in part) by a grant from the Burroughs-Wellcome Fund, by a PHS research grant C-4534 from the NCI, grant DR G-534 from the Damon Runyon Memorial Fund for Cancer Research, and Charles Pfizer and Company.

Fingerprint Dive into the research topics of 'Ion transport and phosphoproteins of human red blood cells'. Together they form a unique fingerprint.

Cite this