Ion- and ligand-exchange chromatography of proteins using porous zirconium oxide supports in organic and inorganic Lewis base eluents

John A. Blackwell, Peter W. Carr

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

The applicability of an eluotropic scale pertaining to the desorption of low molecular weight Lewis base solutes from zirconium oxide is examined for its ability to rationalize the retention of proteins on this substrate. The strongest Lewis base eluents (phosphate and fluoride) are able to bring about elution of nearly all proteins provided that their initial mobile phase concentration almost saturates the eluent's adsorption isotherm. In contrast, weaker Lewis bases such as borate, sulfate and bromide are able to elute only those proteins which are retained primarily by ionic interactions. In weak eluents, proteins that contain a large number of accessible Lewis base sites are not eluted from the support. The effect of ionic strength and a variety of Lewis base eluents were also examined.

Original languageEnglish (US)
Pages (from-to)27-41
Number of pages15
JournalJournal of Chromatography A
Volume596
Issue number1
DOIs
StatePublished - Apr 3 1992

Bibliographical note

Funding Information:
J. A. B. acknowledges financial support from Specialty Adhesives and Chemicals Division and the Leading Edge Academic Program at 3M. This work was also supported in part by grants from the Institute for Advanced Studies in Bioprocess Tech-

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