Involvement of protein acetylation in glucose-induced transcription of a stress-responsive promoter

Bruno P. Lima, Haike Antelmann, Katrin Gronau, Bui Khanh Chi, Dörte Becher, Shaun R. Brinsmade, Alan J. Wolfe

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

In eukaryotes, lysine acetylation is a well-established post-translational modification that has been implicated in virtually all aspects of eukaryotic physiology. Although homologues of the enzymes that catalyse protein acetylation are widely conserved and distributed among bacterial species, not much is known about the impact of protein acetylation on bacterial physiology. Here, we present evidence that the Gcn5-like acetyltransferase YfiQ and the sirtuin deacetylase CobB play crucial roles in the transcription regulation of the periplasmic stress-responsive promoter cpxP when cells of Escherichia coli grow in the presence of glucose, an environment that induces protein acetylation. Under this growth condition, several acetylation sites were detected on three of the RNA polymerase subunits: β, β' and α. We focused on acetylations of the carboxy-terminal domain (CTD) of α because of its relative small size and its limited acetylation. We determined that K298 of α is acetylated in a glucose and YfiQ-dependent manner and that K298 is specifically required for glucose-induced cpxP transcription. Because the αCTD aids in promoter recognition by RNA polymerase, we propose its acetylation may influence bacterial physiology through effects on gene expression.

Original languageEnglish (US)
Pages (from-to)1190-1204
Number of pages15
JournalMolecular Microbiology
Volume81
Issue number5
DOIs
StatePublished - Sep 2011

Fingerprint Dive into the research topics of 'Involvement of protein acetylation in glucose-induced transcription of a stress-responsive promoter'. Together they form a unique fingerprint.

Cite this