Involvement of frizzled-10 in Wnt-7a signaling during chick limb development

Yasuhiko Kawakami, Naoyuki Wada, Shin Ichiro Nishimatsu, Tsutomu Nohno

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

The dorsal ectoderm of the limb bud is known to regulate anterior-posterior patterning as well as dorsalventral patterning during vertebrate limb morphogenesis. Wnt-7a, expressed in the dorsal ectoderm, encodes a key molecule implicated in these events. In the present study, chicken frizzled-10 (Fz-10) encoding a Wnt receptor was used to study mechanisms of Wnt-7a signaling during chick limb patterning, because its expression is restricted to the posterior-distal region of the dorsal limb bud. Fz-10 transcripts colocalize with Sonic hedgehog (Shh) in the dorsal side of stages 18-23 chick limb buds. It was demonstrated that Fz-10 interacts with Wnt-7a to induce synergistically the expression of Wnt-responsive genes, such as Siamois and Xnr3, in Xenopus animal cap assays. In the chick limb bud, Fz-10 expression is regulated by Shh and a signal from the dorsal ectoderm, presumably Wnt-7a, but not by signals from the apical ectodermal ridge. These results suggest that Fz-10 acts as a receptor for Wnt-7a and has a positive effect on Shh expression in the chick limb bud.

Original languageEnglish (US)
Pages (from-to)561-569
Number of pages9
JournalDevelopment Growth and Differentiation
Volume42
Issue number6
DOIs
StatePublished - 2000

Keywords

  • Chick limb development
  • Frizzled-10
  • Sonic hedgehog
  • Wnt-7a

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