Involvement of calcium in mitogenic and immunoglobulin responses in bovine lymphocytes.

D. Atluru, E. D. Sloane, D. W. Johnson, C. C. Muscoplat

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Removal of calcium from cultures of bovine splenic lymphoid cells with ethyleneglycol-bis-(2-amino ethylether)-N,N'-tetraacetic acid (EGTA) caused significant suppression of pokeweed mitogen-induced immunoglobulin (Ig) biosynthesis. It was found that removal of calcium for 48 hours of a 5-day culture significantly altered Ig biosynthesis. Re-addition of calcium after 48 hours did not restore Ig synthesis. The EGTA caused significant (P < 0.05) suppression of DNA synthesis when calcium was removed from concanavalin A- and purified protein derivative-stimulated peripheral blood lymphocyte cultures. Further, removal of calcium for 48 hours of 4-day cultures significantly altered the DNA synthesis in mitogen- and antigen-stimulated cultures.

Original languageEnglish (US)
Pages (from-to)1319-1322
Number of pages4
JournalAmerican journal of veterinary research
Volume41
Issue number8
StatePublished - Aug 1 1980

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