In vitro analysis of partially hydrolyzed guar gum fermentation on identified gut microbiota

Justin Carlson, Trevor Gould, Joanne L Slavin

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Background Prebiotic dietary fibers resist digestion in the upper gastrointestinal tract and allow for stimulation of bacteria in the distal intestine and colon. Stimulation of bacteria among different individuals varies greatly, depending on a wide range of variables. Objective To determine the range of differences in response between individuals, a preclinical in vitro fermentation was conducted with six fecal donors. The primary objective was to compare the fecal microbiota of six individuals at baseline, 12 h and 24 h post-exposure to partially hydrolyzed guar gum (PHGG). Method Fecal donations were collected from six healthy individuals consuming a non-specific Western diet, free of antibiotic treatments in the past year, not affected by any GI diseases and not consuming any probiotic or prebiotic supplements. Fecal samples were exposed to 0.5 g of PHGG and measured for bacterial changes at 0, 12 and 24 h based on 16S rRNA sequencing. Results Parabacteroides increased from 3.48% of sequence reads to 10.62% of sequence reads after 24 h (p = 0.0181) and Bacteroidetes increased from 45.89% of sequence reads to 50.29% of sequence reads (p = 0.0008). Conclusions PHGG stimulates growth of Parabacteroides, a genus of bacteria that have been inversely associated with IBS and ulcerative colitis. PHGG provides stimulation of beneficial Bacteroidetes (Bacteroides and Parabacteroides), which may be correlated with many positive health markers and outcomes. PHGG is a prebiotic dietary fiber that is readily fermentable.

Original languageEnglish (US)
Pages (from-to)60-66
Number of pages7
JournalAnaerobe
Volume42
DOIs
StatePublished - Dec 1 2016

Bibliographical note

Funding Information:
The authors would like to thank Julian Esparza and Jonathon Swan for the laboratory assistance, the University of Minnesota Genomics Center for the guidance and analysis of the samples and The University of Minnesota Doctoral Dissertation Fellowship for support of research.

Publisher Copyright:
© 2016 Elsevier Ltd

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Fermentation
  • Microbiome
  • Microbiota
  • PHGG
  • Prebiotic

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