Investigations on the molecular dosimetry of tobacco-specific N-nitrosamines.

S. S. Hecht; S. G. Carmella; N. Trushin; P. G. Foiles; D. Lin; J. M. Rubin; F. L. Chung

Investigations on the molecular dosimetry of tobacco-specific N-nitrosamines.

S. S. Hecht, S. G. Carmella, N. Trushin, P. G. Foiles, D. Lin, J. M. Rubin, F. L. Chung

Research output: Contribution to journal › Article › peer-review

Abstract

Approaches for assessing molecular dosimetry of 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) and N'-nitrosonornicotine (NNN) in humans by measurement of haemoglobin or DNA adducts are discussed. NNK and NNN form haemoglobin adducts in Fischer 344 rats. Acid or base hydrolysis of the globin gives 4-hydroxy-1-(3-pyridyl)-1-butanone, which can be detected in rat blood up to six weeks after injection of NNK; it may be a useful marker for assessing uptake and metabolic activation of NNK and NNN in tobacco consumers. NNK and its major metabolite, 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanol (NNAI), methylated DNA of rat liver, lung and nasal mucosa to similar extents. NNAI is formed in human tissues from NNK, but immunoassays for O6-methyldeoxyguanosine (O6-medGuo) in exfoliated oral cells from snuff-dippers have been negative. NNK is also expected to form pyridyloxobutyl adducts in DNA; 32P-postlabelling assays for these adducts are being developed and appear to hold promise for detecting NNK- or NNN-DNA adducts in vivo.

Original language	English (US)
Pages (from-to)	423-429
Number of pages	7
Journal	IARC scientific publications
Issue number	84
State	Published - 1987
Externally published	Yes

Find It

Find It at U of M Libraries

Cite this

@article{5393c62c664e4d838dfbe2dd95a0f976,

title = "Investigations on the molecular dosimetry of tobacco-specific N-nitrosamines.",

abstract = "Approaches for assessing molecular dosimetry of 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) and N'-nitrosonornicotine (NNN) in humans by measurement of haemoglobin or DNA adducts are discussed. NNK and NNN form haemoglobin adducts in Fischer 344 rats. Acid or base hydrolysis of the globin gives 4-hydroxy-1-(3-pyridyl)-1-butanone, which can be detected in rat blood up to six weeks after injection of NNK; it may be a useful marker for assessing uptake and metabolic activation of NNK and NNN in tobacco consumers. NNK and its major metabolite, 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanol (NNAI), methylated DNA of rat liver, lung and nasal mucosa to similar extents. NNAI is formed in human tissues from NNK, but immunoassays for O6-methyldeoxyguanosine (O6-medGuo) in exfoliated oral cells from snuff-dippers have been negative. NNK is also expected to form pyridyloxobutyl adducts in DNA; 32P-postlabelling assays for these adducts are being developed and appear to hold promise for detecting NNK- or NNN-DNA adducts in vivo.",

author = "Hecht, {S. S.} and Carmella, {S. G.} and N. Trushin and Foiles, {P. G.} and D. Lin and Rubin, {J. M.} and Chung, {F. L.}",

year = "1987",

language = "English (US)",

pages = "423--429",

journal = "IARC scientific publications",

issn = "0300-5038",

publisher = "IARC",

number = "84",

}

TY - JOUR

T1 - Investigations on the molecular dosimetry of tobacco-specific N-nitrosamines.

AU - Hecht, S. S.

AU - Carmella, S. G.

AU - Trushin, N.

AU - Foiles, P. G.

AU - Lin, D.

AU - Rubin, J. M.

AU - Chung, F. L.

PY - 1987

Y1 - 1987

N2 - Approaches for assessing molecular dosimetry of 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) and N'-nitrosonornicotine (NNN) in humans by measurement of haemoglobin or DNA adducts are discussed. NNK and NNN form haemoglobin adducts in Fischer 344 rats. Acid or base hydrolysis of the globin gives 4-hydroxy-1-(3-pyridyl)-1-butanone, which can be detected in rat blood up to six weeks after injection of NNK; it may be a useful marker for assessing uptake and metabolic activation of NNK and NNN in tobacco consumers. NNK and its major metabolite, 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanol (NNAI), methylated DNA of rat liver, lung and nasal mucosa to similar extents. NNAI is formed in human tissues from NNK, but immunoassays for O6-methyldeoxyguanosine (O6-medGuo) in exfoliated oral cells from snuff-dippers have been negative. NNK is also expected to form pyridyloxobutyl adducts in DNA; 32P-postlabelling assays for these adducts are being developed and appear to hold promise for detecting NNK- or NNN-DNA adducts in vivo.

AB - Approaches for assessing molecular dosimetry of 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) and N'-nitrosonornicotine (NNN) in humans by measurement of haemoglobin or DNA adducts are discussed. NNK and NNN form haemoglobin adducts in Fischer 344 rats. Acid or base hydrolysis of the globin gives 4-hydroxy-1-(3-pyridyl)-1-butanone, which can be detected in rat blood up to six weeks after injection of NNK; it may be a useful marker for assessing uptake and metabolic activation of NNK and NNN in tobacco consumers. NNK and its major metabolite, 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanol (NNAI), methylated DNA of rat liver, lung and nasal mucosa to similar extents. NNAI is formed in human tissues from NNK, but immunoassays for O6-methyldeoxyguanosine (O6-medGuo) in exfoliated oral cells from snuff-dippers have been negative. NNK is also expected to form pyridyloxobutyl adducts in DNA; 32P-postlabelling assays for these adducts are being developed and appear to hold promise for detecting NNK- or NNN-DNA adducts in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0023495374&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023495374&partnerID=8YFLogxK

M3 - Article

C2 - 3679413

AN - SCOPUS:0023495374

SN - 0300-5038

SP - 423

EP - 429

JO - IARC scientific publications

JF - IARC scientific publications

IS - 84

ER -

Investigations on the molecular dosimetry of tobacco-specific N-nitrosamines.

Abstract

Find It

Other files and links

Fingerprint

Cite this