TY - GEN
T1 - Investigation of the mechanism of contact guidance of neurite growth cones in magnetically-aligned collagen gels
AU - Dubey, Naren
AU - Letourneau, Paul C.
AU - Tranquillo, Robert T
PY - 1999
Y1 - 1999
N2 - We have studied the adhesion anisotropy hypothesis of neuronal contact guidance by culturing PC12 cells in magnetically-aligned collagen gels. Neurite contact guidance was compared between aligned collagen gels and gels aligned to the same degree but subsequently equilibrated in a solution of monoclonal anti-rat α1 integrin antibody that is known to inhibit rat PC12 neuritogenesis. Modulation of integrin binding would not affect the anisotropic mechanical properties of the aligned collagen network, but it would affect growth cone adhesion, which is anisotropic due to the aligned collagen fibrils. Contact guidance was quantified by measuring the length and orientation of neurite projection 48 hours after culture. We found no difference in the contact guidance response, implying that the contact guidance mechanism is not primarily due to anisotropic adhesion. Experiments are currently underway to investigate a mechanical anisotropy mechanism.
AB - We have studied the adhesion anisotropy hypothesis of neuronal contact guidance by culturing PC12 cells in magnetically-aligned collagen gels. Neurite contact guidance was compared between aligned collagen gels and gels aligned to the same degree but subsequently equilibrated in a solution of monoclonal anti-rat α1 integrin antibody that is known to inhibit rat PC12 neuritogenesis. Modulation of integrin binding would not affect the anisotropic mechanical properties of the aligned collagen network, but it would affect growth cone adhesion, which is anisotropic due to the aligned collagen fibrils. Contact guidance was quantified by measuring the length and orientation of neurite projection 48 hours after culture. We found no difference in the contact guidance response, implying that the contact guidance mechanism is not primarily due to anisotropic adhesion. Experiments are currently underway to investigate a mechanical anisotropy mechanism.
UR - https://www.scopus.com/pages/publications/0033354110
UR - https://www.scopus.com/pages/publications/0033354110#tab=citedBy
M3 - Conference contribution
AN - SCOPUS:0033354110
SN - 0780356756
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 39
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PB - IEEE
T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
Y2 - 13 October 1999 through 16 October 1999
ER -