Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community

Lea Ellegaard-Petersen; Henriette Selck; Anders Priemé; Daniel Salvito; Valery Forbes

doi:10.1007/s10646-010-0486-z

Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community

Lea Ellegaard-Petersen, Henriette Selck, Anders Priemé, Daniel Salvito, Valery Forbes

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3 Scopus citations

Abstract

The fate of the fragrance material, acetyl cedrene (AC), in sediment was examined in a 16 day laboratory experiment using the sediment microbial community subjected to the following treatments: AC (nominal concentration; 0 and 50 μg g^-1 dw sediment) and macrofaunal worms (with/without Capitella teleta (formerly Capitella sp. I)). Furthermore effects of AC on microbial respiration in the system were determined by examining CO ₂ flux. T-RFLP (terminal restriction fragment length polymorphism) was used to analyze PCR (polymerase chain reaction) amplified 16S DNA gene fragments from the sediments to detect changes in the structure and diversity of the bacterial community. In addition, survival of C. teleta in sediment was determined. Lastly, we examined how the interactions between microbes and C. teleta in the sediment affected the above-mentioned parameters. The results showed that there was an interaction between worm treatment and time of sampling on the loss of AC from the sediment. This was caused by AC loss initially being fastest in the sediment with C. teleta present, but at experimental termination there was no significant difference between the two treatments (i.e., with/without worms) in the amount of AC remaining in the sediment. Survival of C. teleta was significantly reduced by AC at experimental termination, but neither microbial respiration nor structure and diversity of the bacterial community were significantly affected.

Original language	English (US)
Pages (from-to)	1046-1058
Number of pages	13
Journal	Ecotoxicology
Volume	19
Issue number	6
DOIs	https://doi.org/10.1007/s10646-010-0486-z
State	Published - Aug 2010

Bibliographical note

Funding Information:
Acknowledgments The authors thank K. Vestberg for assistance with the molecular work and P. Christensen for assistance with the fragrance extraction method. Our thanks also go to G. Banta and A.B. Faarborg for guidance on the CO2 measurement. Lastly, we are grateful to L.D. Rasmussen, National Veterinary Institute, DTU for running the T-RFLP analysis. This work was partly funded by a grant from the Research Institute for Fragrance Materials.

Keywords

Bacterial community
Ecological risk
Fragrance materials
Microbe-macrofauna interactions
Sediment contamination
T-RFLP

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10.1007/s10646-010-0486-z

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title = "Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community",

abstract = "The fate of the fragrance material, acetyl cedrene (AC), in sediment was examined in a 16 day laboratory experiment using the sediment microbial community subjected to the following treatments: AC (nominal concentration; 0 and 50 μg g-1 dw sediment) and macrofaunal worms (with/without Capitella teleta (formerly Capitella sp. I)). Furthermore effects of AC on microbial respiration in the system were determined by examining CO 2 flux. T-RFLP (terminal restriction fragment length polymorphism) was used to analyze PCR (polymerase chain reaction) amplified 16S DNA gene fragments from the sediments to detect changes in the structure and diversity of the bacterial community. In addition, survival of C. teleta in sediment was determined. Lastly, we examined how the interactions between microbes and C. teleta in the sediment affected the above-mentioned parameters. The results showed that there was an interaction between worm treatment and time of sampling on the loss of AC from the sediment. This was caused by AC loss initially being fastest in the sediment with C. teleta present, but at experimental termination there was no significant difference between the two treatments (i.e., with/without worms) in the amount of AC remaining in the sediment. Survival of C. teleta was significantly reduced by AC at experimental termination, but neither microbial respiration nor structure and diversity of the bacterial community were significantly affected.",

keywords = "Bacterial community, Ecological risk, Fragrance materials, Microbe-macrofauna interactions, Sediment contamination, T-RFLP",

author = "Lea Ellegaard-Petersen and Henriette Selck and Anders Priem{\'e} and Daniel Salvito and Valery Forbes",

note = "Funding Information: Acknowledgments The authors thank K. Vestberg for assistance with the molecular work and P. Christensen for assistance with the fragrance extraction method. Our thanks also go to G. Banta and A.B. Faarborg for guidance on the CO2 measurement. Lastly, we are grateful to L.D. Rasmussen, National Veterinary Institute, DTU for running the T-RFLP analysis. This work was partly funded by a grant from the Research Institute for Fragrance Materials.",

year = "2010",

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doi = "10.1007/s10646-010-0486-z",

language = "English (US)",

volume = "19",

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T1 - Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community

AU - Ellegaard-Petersen, Lea

AU - Selck, Henriette

AU - Priemé, Anders

AU - Salvito, Daniel

AU - Forbes, Valery

N1 - Funding Information: Acknowledgments The authors thank K. Vestberg for assistance with the molecular work and P. Christensen for assistance with the fragrance extraction method. Our thanks also go to G. Banta and A.B. Faarborg for guidance on the CO2 measurement. Lastly, we are grateful to L.D. Rasmussen, National Veterinary Institute, DTU for running the T-RFLP analysis. This work was partly funded by a grant from the Research Institute for Fragrance Materials.

PY - 2010/8

Y1 - 2010/8

N2 - The fate of the fragrance material, acetyl cedrene (AC), in sediment was examined in a 16 day laboratory experiment using the sediment microbial community subjected to the following treatments: AC (nominal concentration; 0 and 50 μg g-1 dw sediment) and macrofaunal worms (with/without Capitella teleta (formerly Capitella sp. I)). Furthermore effects of AC on microbial respiration in the system were determined by examining CO 2 flux. T-RFLP (terminal restriction fragment length polymorphism) was used to analyze PCR (polymerase chain reaction) amplified 16S DNA gene fragments from the sediments to detect changes in the structure and diversity of the bacterial community. In addition, survival of C. teleta in sediment was determined. Lastly, we examined how the interactions between microbes and C. teleta in the sediment affected the above-mentioned parameters. The results showed that there was an interaction between worm treatment and time of sampling on the loss of AC from the sediment. This was caused by AC loss initially being fastest in the sediment with C. teleta present, but at experimental termination there was no significant difference between the two treatments (i.e., with/without worms) in the amount of AC remaining in the sediment. Survival of C. teleta was significantly reduced by AC at experimental termination, but neither microbial respiration nor structure and diversity of the bacterial community were significantly affected.

AB - The fate of the fragrance material, acetyl cedrene (AC), in sediment was examined in a 16 day laboratory experiment using the sediment microbial community subjected to the following treatments: AC (nominal concentration; 0 and 50 μg g-1 dw sediment) and macrofaunal worms (with/without Capitella teleta (formerly Capitella sp. I)). Furthermore effects of AC on microbial respiration in the system were determined by examining CO 2 flux. T-RFLP (terminal restriction fragment length polymorphism) was used to analyze PCR (polymerase chain reaction) amplified 16S DNA gene fragments from the sediments to detect changes in the structure and diversity of the bacterial community. In addition, survival of C. teleta in sediment was determined. Lastly, we examined how the interactions between microbes and C. teleta in the sediment affected the above-mentioned parameters. The results showed that there was an interaction between worm treatment and time of sampling on the loss of AC from the sediment. This was caused by AC loss initially being fastest in the sediment with C. teleta present, but at experimental termination there was no significant difference between the two treatments (i.e., with/without worms) in the amount of AC remaining in the sediment. Survival of C. teleta was significantly reduced by AC at experimental termination, but neither microbial respiration nor structure and diversity of the bacterial community were significantly affected.

KW - Bacterial community

KW - Ecological risk

KW - Fragrance materials

KW - Microbe-macrofauna interactions

KW - Sediment contamination

KW - T-RFLP

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SN - 0963-9292

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EP - 1058

JO - Ecotoxicology

JF - Ecotoxicology

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ER -

Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community

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