Investigation of subzero water transport properties for isolated hepatocytes and hepatocytes cultured in spheroids

T. Brian Darr, Allison Hubel

Research output: Contribution to conferencePaper

3 Scopus citations

Abstract

The water transport properties are investigated in the presence of external ice, isolated pig hepatocytes, and cultured rat hepatocyte spheroids. The water permeability parameters can be combined with models for internal ice formation to estimate optimum freezing conditions for bulk cryogenic storage of isolated hepatocytes. A cryomicroscopy system is used to perform the freezing investigations. The results from these studies will be used to determine protocols for the cryopreservation of hepatocytes or spheroids for use in a bioartificial liver.

Original languageEnglish (US)
Pages269-270
Number of pages2
StatePublished - Jan 1 1995
EventProceedings of the 1995 Bioengineering Conference - Beever Creek, CO, USA
Duration: Jun 28 1995Jul 2 1995

Other

OtherProceedings of the 1995 Bioengineering Conference
CityBeever Creek, CO, USA
Period6/28/957/2/95

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    Darr, T. B., & Hubel, A. (1995). Investigation of subzero water transport properties for isolated hepatocytes and hepatocytes cultured in spheroids. 269-270. Paper presented at Proceedings of the 1995 Bioengineering Conference, Beever Creek, CO, USA, .