Investigation of properties of chemically cross-linked silk nanofibrous mat as a nerve guide

Maryam Ebrahimi; Jafar Ai; Esmaeil Biazar; Reza Faridi-Majidi; Jamshid Hajati; Somayeh Ebrahimi-Barough; Saeed Heidari K

doi:10.1080/10667857.2017.1317065

Investigation of properties of chemically cross-linked silk nanofibrous mat as a nerve guide

Maryam Ebrahimi
, Jafar Ai
, Esmaeil Biazar
, Reza Faridi-Majidi
, Jamshid Hajati
, Somayeh Ebrahimi-Barough
, Saeed Heidari K

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The aim of this study was to modify a nanofibrous silk mate with a chemically cross-linker. The nanofibrous mats designed by electrospinning method, then were modified using chemically cross-linker of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) in the presence of N-hydroxysuccinimide (NHS). The samples evaluated by microscopic, mechanical analyses, and cell culture assays with Schwann cells. Results of analyses showed a good resilience and compliance with chemically cross-linker. Cellular experiments showed a suitable cell adhesion and growth for the cross-linked mats without any toxicity. This modified mat appears to have the right organisation for testing in vivo special nerve tissue regeneration.

Original language	English (US)
Pages (from-to)	551-559
Number of pages	9
Journal	Materials Technology
Volume	32
Issue number	9
DOIs	https://doi.org/10.1080/10667857.2017.1317065
State	Published - Jul 29 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 Informa UK Limited, trading as Taylor & Francis Group.

Keywords

1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)
cell culture
Electrospinning
nanofibrous silk
Schwann cell

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10.1080/10667857.2017.1317065

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title = "Investigation of properties of chemically cross-linked silk nanofibrous mat as a nerve guide",

abstract = "The aim of this study was to modify a nanofibrous silk mate with a chemically cross-linker. The nanofibrous mats designed by electrospinning method, then were modified using chemically cross-linker of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) in the presence of N-hydroxysuccinimide (NHS). The samples evaluated by microscopic, mechanical analyses, and cell culture assays with Schwann cells. Results of analyses showed a good resilience and compliance with chemically cross-linker. Cellular experiments showed a suitable cell adhesion and growth for the cross-linked mats without any toxicity. This modified mat appears to have the right organisation for testing in vivo special nerve tissue regeneration.",

keywords = "1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), cell culture, Electrospinning, nanofibrous silk, Schwann cell",

author = "Maryam Ebrahimi and Jafar Ai and Esmaeil Biazar and Reza Faridi-Majidi and Jamshid Hajati and Somayeh Ebrahimi-Barough and \{Heidari K\}, Saeed",

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T1 - Investigation of properties of chemically cross-linked silk nanofibrous mat as a nerve guide

AU - Ebrahimi, Maryam

AU - Ai, Jafar

AU - Biazar, Esmaeil

AU - Faridi-Majidi, Reza

AU - Hajati, Jamshid

AU - Ebrahimi-Barough, Somayeh

AU - Heidari K, Saeed

PY - 2017/7/29

Y1 - 2017/7/29

N2 - The aim of this study was to modify a nanofibrous silk mate with a chemically cross-linker. The nanofibrous mats designed by electrospinning method, then were modified using chemically cross-linker of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) in the presence of N-hydroxysuccinimide (NHS). The samples evaluated by microscopic, mechanical analyses, and cell culture assays with Schwann cells. Results of analyses showed a good resilience and compliance with chemically cross-linker. Cellular experiments showed a suitable cell adhesion and growth for the cross-linked mats without any toxicity. This modified mat appears to have the right organisation for testing in vivo special nerve tissue regeneration.

AB - The aim of this study was to modify a nanofibrous silk mate with a chemically cross-linker. The nanofibrous mats designed by electrospinning method, then were modified using chemically cross-linker of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) in the presence of N-hydroxysuccinimide (NHS). The samples evaluated by microscopic, mechanical analyses, and cell culture assays with Schwann cells. Results of analyses showed a good resilience and compliance with chemically cross-linker. Cellular experiments showed a suitable cell adhesion and growth for the cross-linked mats without any toxicity. This modified mat appears to have the right organisation for testing in vivo special nerve tissue regeneration.

KW - 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)

KW - cell culture

KW - Electrospinning

KW - nanofibrous silk

KW - Schwann cell

UR - https://www.scopus.com/pages/publications/85018514176

UR - https://www.scopus.com/pages/publications/85018514176#tab=citedBy

U2 - 10.1080/10667857.2017.1317065

DO - 10.1080/10667857.2017.1317065

M3 - Article

AN - SCOPUS:85018514176

SN - 1066-7857

VL - 32

SP - 551

EP - 559

JO - Materials Technology

JF - Materials Technology

IS - 9

ER -

Investigation of properties of chemically cross-linked silk nanofibrous mat as a nerve guide

Abstract

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Keywords

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