Investigation of novel cold atmospheric plasma sources and their impact on the structural and functional characteristics of pea protein

Fan Bu, Samira Feyzi, Gaurav Nayak, Qingqing Mao, Vighneswara Siva Santosh Kumar Kondeti, Peter Bruggeman, Chi Chen, Baraem P. Ismail

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The impact of three cold atmospheric plasma (CAP) sources, atmospheric pressure plasma jet (APPJ), two- dimension dielectric barrier discharge (2D-DBD), and nanosecond pulsed discharge (ns-pulsed) on the structure, functionality, and amino acid composition of pea protein was evaluated. Different plasma sources and associated reactive species resulted in protein denaturation, increased surface hydrophobicity, formation of soluble aggregates mostly by disulfide linkages, and changes in secondary structures. Enhancement in surface properties, presence of soluble aggregates, and increase in β-sheet contributed to significant improvement in gelation and emulsification. Enhanced emulsion stability was attributed to relatively small droplet sizes and high surface-charge. Differences among CAP-treated samples were attributed to differences in fluence and composition of plasma-produced reactive species. While all three plasma treatments could be appreciable functionalization approaches, 2D-DBD (Ar + O2) treatment for 30 min had insignificant effect on the amino acid composition.

Original languageEnglish (US)
Article number103248
JournalInnovative Food Science and Emerging Technologies
Volume83
DOIs
StatePublished - Jan 2023

Bibliographical note

Funding Information:
This project was funded by the Plant Protein Innovation Center (PPIC). FTIR-ATR data collection was performed at the Characterization Facility, University of Minnesota , which receives partial support from the NSF through the MRSEC (Award Number DMR-2011401 ) and the NNCI (Award Number ECCS-2025124 ) programs. CLSM images were captured and analyzed with the support of the resources and staff at the University Imaging Centers (UIC) at University of Minnesota ( SCR-020997 ).

Funding Information:
This project was funded by the Plant Protein Innovation Center (PPIC). FTIR-ATR data collection was performed at the Characterization Facility, University of Minnesota, which receives partial support from the NSF through the MRSEC (Award Number DMR-2011401) and the NNCI (Award Number ECCS-2025124) programs. CLSM images were captured and analyzed with the support of the resources and staff at the University Imaging Centers (UIC) at University of Minnesota (SCR-020997).

Publisher Copyright:
© 2022 Elsevier Ltd

Keywords

  • 2D-dielectric barrier discharge
  • Atmospheric pressure plasma jet
  • Cold atmospheric plasma
  • Nanosecond pulsed discharge
  • Pea protein
  • Structure and functionality

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