Abstract
The impact of three cold atmospheric plasma (CAP) sources, atmospheric pressure plasma jet (APPJ), two- dimension dielectric barrier discharge (2D-DBD), and nanosecond pulsed discharge (ns-pulsed) on the structure, functionality, and amino acid composition of pea protein was evaluated. Different plasma sources and associated reactive species resulted in protein denaturation, increased surface hydrophobicity, formation of soluble aggregates mostly by disulfide linkages, and changes in secondary structures. Enhancement in surface properties, presence of soluble aggregates, and increase in β-sheet contributed to significant improvement in gelation and emulsification. Enhanced emulsion stability was attributed to relatively small droplet sizes and high surface-charge. Differences among CAP-treated samples were attributed to differences in fluence and composition of plasma-produced reactive species. While all three plasma treatments could be appreciable functionalization approaches, 2D-DBD (Ar + O2) treatment for 30 min had insignificant effect on the amino acid composition.
Original language | English (US) |
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Article number | 103248 |
Journal | Innovative Food Science and Emerging Technologies |
Volume | 83 |
DOIs | |
State | Published - Jan 2023 |
Bibliographical note
Funding Information:This project was funded by the Plant Protein Innovation Center (PPIC). FTIR-ATR data collection was performed at the Characterization Facility, University of Minnesota , which receives partial support from the NSF through the MRSEC (Award Number DMR-2011401 ) and the NNCI (Award Number ECCS-2025124 ) programs. CLSM images were captured and analyzed with the support of the resources and staff at the University Imaging Centers (UIC) at University of Minnesota ( SCR-020997 ).
Funding Information:
This project was funded by the Plant Protein Innovation Center (PPIC). FTIR-ATR data collection was performed at the Characterization Facility, University of Minnesota, which receives partial support from the NSF through the MRSEC (Award Number DMR-2011401) and the NNCI (Award Number ECCS-2025124) programs. CLSM images were captured and analyzed with the support of the resources and staff at the University Imaging Centers (UIC) at University of Minnesota (SCR-020997).
Publisher Copyright:
© 2022 Elsevier Ltd
Keywords
- 2D-dielectric barrier discharge
- Atmospheric pressure plasma jet
- Cold atmospheric plasma
- Nanosecond pulsed discharge
- Pea protein
- Structure and functionality