Investigation of calcium mechanotransduction by quasi 3-D microfiber mechanical stimulation of cells

Warren C. Ruder, Erica D Pratt, Metin Sitti, Philip R. Leduc, James F. Antaki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Fibroblast cells mechanically interact with their environment through several mechanisms, including the formation of focal adhesion complexes (FACs) with adjacent ligands. A quasi-3D (Q3D) experimental device was devised to investigate chemo-mechanical stimulation of these cells. It employed a novel microscopic fixture featuring micropolymer arrays positioned just above a cellular monolayer, enabling mechanical stimulation through dorsal attachment to FACs. This protocol demonstrated the ability to preserve the normal stellate morphology of fibroblasts. Live-cell imaging during mechanical stimulation revealed robust calcium elevation in cells interacting with the Q3D device. This system provides a simple alternative to existing 3D culture methods yet allows dynamic, real-time mechanical probing of cells in a physiologically significant environment as compared to optical or magnetic tweezers.

Original languageEnglish (US)
Title of host publicationProceedings of the ASME Summer Bioengineering Conference, SBC2008
Pages1049-1050
Number of pages2
EditionPART B
StatePublished - Dec 1 2009
Externally publishedYes
Event10th ASME Summer Bioengineering Conference, SBC2008 - Marco Island, FL, United States
Duration: Jun 25 2008Jun 29 2008

Other

Other10th ASME Summer Bioengineering Conference, SBC2008
CountryUnited States
CityMarco Island, FL
Period6/25/086/29/08

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