@inproceedings{10648d5e0f5847059a818175041c6e48,
title = "Investigation of calcium mechanotransduction by quasi 3-D microfiber mechanical stimulation of cells",
abstract = "Fibroblast cells mechanically interact with their environment through several mechanisms, including the formation of focal adhesion complexes (FACs) with adjacent ligands. A quasi-3D (Q3D) experimental device was devised to investigate chemo-mechanical stimulation of these cells. It employed a novel microscopic fixture featuring micropolymer arrays positioned just above a cellular monolayer, enabling mechanical stimulation through dorsal attachment to FACs. This protocol demonstrated the ability to preserve the normal stellate morphology of fibroblasts. Live-cell imaging during mechanical stimulation revealed robust calcium elevation in cells interacting with the Q3D device. This system provides a simple alternative to existing 3D culture methods yet allows dynamic, real-time mechanical probing of cells in a physiologically significant environment as compared to optical or magnetic tweezers.",
author = "Ruder, {Warren C.} and Pratt, {Erica D.} and Metin Sitti and Leduc, {Philip R.} and Antaki, {James F.}",
year = "2009",
language = "English (US)",
isbn = "9780791843215",
series = "Proceedings of the ASME Summer Bioengineering Conference, SBC2008",
number = "PART B",
pages = "1049--1050",
booktitle = "Proceedings of the ASME Summer Bioengineering Conference, SBC2008",
edition = "PART B",
note = "10th ASME Summer Bioengineering Conference, SBC2008 ; Conference date: 25-06-2008 Through 29-06-2008",
}