Investigation of Azospirillum brasilense Inoculation and Silicon Application on Corn Yield Responses

Fernando Shintate Galindo, Paulo Humberto Pagliari, Willian Lima Rodrigues, Maikon Richer de Azambuja Pereira, Salatiér Buzetti, Marcelo Carvalho Minhoto Teixeira Filho

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


New management practices are needed to help improve the inoculation efficiency of plant growth-promoting bacteria (PGPB). The use of silicon (Si) combined with PGPB can be a useful and sustainable strategy to enhance cereal development under tropical conditions. This research was developed to investigate inoculation methods with Azospirillum brasilense combined with Si application as a soil amendment in corn. The effects of Si application and A. brasilense inoculation on plant development and yield were tested on corn for two consecutive years. The study was set up in a Rhodic Hapludox under a no-till system in a completely randomized block design with four replicates. Treatments were tested in a full factorial design (triple factorial 4 × 2 × 2) and included (i) 4 inoculation methods (control, seed, in-furrow, and leaf), (ii) two lime sources (calcium and magnesium silicate and dolomitic limestone), and (iii) two study years (2015/2016 and 2016/2017). Our results showed that seed and in-furrow inoculation were more effective in promoting corn development and greater yield, with an increase of 10.6 and 7.3% in corn grain yield, respectively. Silicate application combined with seed inoculation increased shoot N uptake and root biomass. Also, Si application increased leaf chlorophyll index, stem diameter, and had similar effects on soil properties compared with limestone. Our results showed positive effects on corn growth and productivity as a result of inoculation, while the potential benefits of Si use were less evident.

Original languageEnglish (US)
Pages (from-to)2406-2418
Number of pages13
JournalJournal of Soil Science and Plant Nutrition
Issue number4
StatePublished - Dec 2020

Bibliographical note

Funding Information:
This study was funded by the FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) (grant number 17/06002-6) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) (award number 312359/2017-9).

Publisher Copyright:
© 2020, Sociedad Chilena de la Ciencia del Suelo.


  • Beneficial element
  • Calcium and magnesium silicate
  • Inoculation methods
  • Plant growth-promoting bacteria
  • Plant nutrition
  • Zea mays L


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