Investigation and Conformational Analysis of Fluorinated Nucleoside Antibiotics Targeting Siderophore Biosynthesis

Surendra Dawadi, Kishore Viswanathan, Helena I. Boshoff, Clifton E. Barry, Courtney C. Aldrich

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

(Chemical Equation Presented) Antibiotic resistance represents one of the greatest threats to public health. The adenylation inhibitor 5′-O-[N-(salicyl)sulfamoyl]adenosine (SAL-AMS) is the archetype for a new class of nucleoside antibiotics that target iron acquisition in pathogenic microorganisms and is especially effective against Mycobacterium tuberculosis, the causative agent of tuberculosis. Strategic incorporation of fluorine at the 2′ and 3′ positions of the nucleoside was performed by direct fluorination to enhance activity and improve drug disposition properties. The resulting SAL-AMS analogues were comprehensively assessed for biochemical potency, whole-cell antitubercular activity, and in vivo pharmacokinetic parameters. Conformational analysis suggested a strong preference of fluorinated sugar rings for either a 2′-endo, 3′-exo (South), or a 3′-endo,2′-exo (North) conformation. The structure-activity relationships revealed a strong conformational bias for the C3′-endo conformation to maintain potent biochemical and whole-cell activity, whereas improved pharmacokinetic properties were associated with the C2′-endo conformation.

Original languageEnglish (US)
Pages (from-to)4835-4850
Number of pages16
JournalJournal of Organic Chemistry
Volume80
Issue number10
DOIs
StatePublished - May 15 2015

Bibliographical note

Publisher Copyright:
© 2015 American Chemical Society.

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