Inventories of naive and tolerant mouse CD4 T cell repertoires reveal a hierarchy of deleted and diverted T cell receptors

Tobias Hassler, Emanuel Urmann, Sebastian Teschner, Christine Federle, Thamotharampillai Dileepan, Kilian Schober, Marc K. Jenkins, Dirk H. Busch, Maria Hinterberger, Ludger Klein

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Deletion or Treg cell differentiation are alternative fates of autoreactive MHCII-restricted thymocytes. How these different modes of tolerance determine the size and composition of polyclonal cohorts of autoreactive T cells with shared specificity is poorly understood. We addressed how tolerance to a naturally expressed autoantigen of the central nervous system shapes the CD4 T cell repertoire. Specific cells in the tolerant peripheral repertoire either were Foxp3+ or displayed anergy hallmarks and, surprisingly, were at least as frequent as in the nontolerant repertoire. Despite this apparent lack of deletional tolerance, repertoire inventories uncovered that some T cell receptors (TCRs) were lost from the CD4 T cell pool, whereas others mediated Treg cell differentiation. The antigen responsiveness of these TCRs supported an affinity model of central tolerance. Importantly, the contribution of different diverter TCRs to the nascent thymic Treg cell population reflected their antigen reactivity rather than their frequency among precursors. This reveals a multilayered TCR hierarchy in CD4 T cell tolerance that separates deleted and diverted TCRs and assures that the Treg cell compartment is filled with cells of maximal permissive antigen reactivity.

Original languageEnglish (US)
Pages (from-to)18537-18543
Number of pages7
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number37
DOIs
StatePublished - Sep 10 2019

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T-Cell Antigen Receptor
Regulatory T-Lymphocytes
T-Lymphocytes
Equipment and Supplies
Cell Differentiation
Central Tolerance
Antigens
Autoantigens
Thymocytes
Central Nervous System
Population

Keywords

  • Clona
  • Clonal diversion
  • Deletion
  • MHC class II tetramer
  • Regulatory T cell
  • T cell tolerance

PubMed: MeSH publication types

  • Journal Article

Cite this

Inventories of naive and tolerant mouse CD4 T cell repertoires reveal a hierarchy of deleted and diverted T cell receptors. / Hassler, Tobias; Urmann, Emanuel; Teschner, Sebastian; Federle, Christine; Dileepan, Thamotharampillai; Schober, Kilian; Jenkins, Marc K.; Busch, Dirk H.; Hinterberger, Maria; Klein, Ludger.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, No. 37, 10.09.2019, p. 18537-18543.

Research output: Contribution to journalArticle

Hassler, Tobias ; Urmann, Emanuel ; Teschner, Sebastian ; Federle, Christine ; Dileepan, Thamotharampillai ; Schober, Kilian ; Jenkins, Marc K. ; Busch, Dirk H. ; Hinterberger, Maria ; Klein, Ludger. / Inventories of naive and tolerant mouse CD4 T cell repertoires reveal a hierarchy of deleted and diverted T cell receptors. In: Proceedings of the National Academy of Sciences of the United States of America. 2019 ; Vol. 116, No. 37. pp. 18537-18543.
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