Intravital microscopy identifies selectins that regulate T cell traffic into allografts

Thomas R. Jones, Nozomu Shirasugi, Andrew B. Adams, Thomas C. Pearson, Christian P. Larsen

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


T cell homing to sites of injury and inflammation is a critical step for adaptive immune responses. While much has been learned regarding T cell homing to lymphoid tissues, few studies have directly observed trafficking events during an effector response. In this study, we developed a model that uses intravital fluorescence videomicroscopy to determine the molecules critical to T cell rolling within skin allograft microvasculature during the effector phase of the rejection response. Additional studies were performed to quantify T cell infiltrates as rejection progressed. We found that P-selectin and E-selectin expressed on postcapillary venules play overlapping roles in the recruitment of activated T cells in a SCID reconstitution model of skin graft rejection and are important in T cell accumulation at the graft site. Surprisingly, we also found that naive T cells are recruited and accumulate via constitutive T cell L-selectin and upregulated L-selectin ligands on rejecting allograft vasculature. These data indicated that a specific retinue of molecules is upregulated during the rejection response, and they suggest potential future therapeutic targets.

Original languageEnglish (US)
Pages (from-to)1714-1723
Number of pages10
JournalJournal of Clinical Investigation
Issue number11
StatePublished - Dec 2003
Externally publishedYes


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