Intraspinal transplantation and modulation of donor neuron electrophysiological activity

Kun Ze Lee, Michael A. Lane, Brendan J. Dougherty, Lynne M. Mercier, Milapjit S. Sandhu, Justin C. Sanchez, Paul J. Reier, David D. Fuller

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Rat fetal spinal cord (FSC) tissue, naturally enriched with interneuronal progenitors, was introduced into high cervical, hemi-resection (Hx) lesions. Electrophysiological analyses were conducted to determine if such grafts exhibit physiologically-patterned neuronal activity and if stimuli which increase respiratory motor output also alter donor neuron bursting. Three months following transplantation, the bursting activity of FSC neurons and the contralateral phrenic nerve were recorded in anesthetized rats during a normoxic baseline period and brief respiratory challenges. Spontaneous neuronal activity was detected in 80% of the FSC transplants, and autocorrelation of action potential spikes revealed distinct correlogram peaks in 87% of neurons. At baseline, the average discharge frequency of graft neurons was 13.0 ± 1.7. Hz, and discharge frequency increased during a hypoxic respiratory challenge (p. <. 0.001). Parallel studies in unanesthetized rats showed that FSC tissue recipients had larger inspiratory tidal volumes during brief hypoxic exposures (p. <. 0.05 vs. C2Hx rats). Anatomical connectivity was explored in additional graft recipients by injecting a transsynaptic retrograde viral tracer (pseudorabies virus, PRV) directly into matured transplants. Neuronal labeling occurred throughout graft tissues and also in the host spinal cord and brainstem nuclei, including those associated with respiratory control. These results underscore the neuroplastic potential of host-graft interactions and training approaches to enhance functional integration within targeted spinal circuitry.

Original languageEnglish (US)
Pages (from-to)47-57
Number of pages11
JournalExperimental Neurology
StatePublished - Jan 2014

Bibliographical note

Funding Information:
Support for this work was provided by grants from the National Institutes of Health (NIH) : R21 HL104294-01 (DDF) and P40RR018604 to Dr. Card who supplied the PRV. Support was also provided by the Oscar and Anne Lackner Chair in Medicine (PJR), the Paralyzed Veterans of America (MAL & KZL), and the Brain and Spinal Cord Injury Research Trust Fund, University of Florida (DDF, PJR).


  • Cervical spinal cord injury
  • Fetal spinal cord
  • Hypoxia
  • Respiratory recovery
  • Spinal cord repair
  • Transplantation


Dive into the research topics of 'Intraspinal transplantation and modulation of donor neuron electrophysiological activity'. Together they form a unique fingerprint.

Cite this