Intracellular pH in single Saccharomyces cerevisiae cells

B. J. Hernlem, F. Srienc

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Saccharomyces cerevisiae cells can be stained with the pH dependent fluorochrome BCECF, to monitor shifts in intracellular pH in individual cells using flow cytometry. Cells stained in the presence of ethanol were found to be stained as much as 100 times more intensely as cells without ethanol. Cells that have been starved for the carbon source showed a significant shift in intracellular pH upon refeeding with a metabolizable carbon source within 3 minutes.

Original languageEnglish (US)
Pages (from-to)79-84
Number of pages6
JournalBiotechnology Techniques
Volume3
Issue number2
DOIs
StatePublished - Mar 1 1989

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