Intracellular Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in buccal epithelial cells collected from human subjects

Joel D Rudney, R. Chen, G. J. Sedgewick

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

The mouth may provide an accessible model for studying bacterial interactions with human cells in vivo. Using fluorescent in situ hybridization and laser scanning confocal microscopy, we found that human buccal epithelial cells from 23 of 24 subjects were infected with intracellular bacteria, including the periodontal pathogens Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis, as well as other species which have yet to be identified. Buccal cell invasion may allow fastidious anaerobes to establish themselves in aerobic sites that otherwise present an unfavorable environment. Exfoliated buccal epithelial cells might provide a protected route for bacterial transmission between different oral sites within and between hosts.

Original languageEnglish (US)
Pages (from-to)2700-2707
Number of pages8
JournalInfection and immunity
Volume69
Issue number4
DOIs
StatePublished - Apr 5 2001

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Aggregatibacter actinomycetemcomitans
Porphyromonas gingivalis
Cheek
Epithelial Cells
Fluorescence In Situ Hybridization
Confocal Microscopy
Mouth
Bacteria

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Intracellular Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in buccal epithelial cells collected from human subjects. / Rudney, Joel D; Chen, R.; Sedgewick, G. J.

In: Infection and immunity, Vol. 69, No. 4, 05.04.2001, p. 2700-2707.

Research output: Contribution to journalArticle

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