Intra- and inter-molecular interactions of human galectin-3 : assessment by full-assignment-based NMR

Hans Ippel, Michelle C Miller, Sabine Vértesy, Yi Zhang, F Javier Cañada, Dennis Suylen, Kimiko Umemoto, Cecilia Romanò, Tilman Hackeng, Guihua Tai

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28 Scopus citations

Abstract

Galectin-3 is an adhesion/growth-regulatory protein with a modular design comprising an N-terminal tail (NT, residues 1-111) and the conserved carbohydrate recognition domain (CRD, residues 112-250). The chimera-type galectin interacts with both glycan and peptide motifs. Complete (13)C/(15)N-assignment of the human protein makes NMR-based analysis of its structure beyond the CRD possible. Using two synthetic NT polypeptides covering residues 1-50 and 51-107, evidence for transient secondary structure was found with helical conformation from residues 5 to 15 as well as proline-mediated, multi-turn structure from residues 18 to 32 and around PGAYP repeats. Intramolecular interactions occur between the CRD F-face (the 5-stranded β-sheet behind the canonical carbohydrate-binding 6-stranded β-sheet of the S-face) and NT in full-length galectin-3, with the sequence P(23)GAW(26) … P(37)GASYPGAY(45) defining the primary binding epitope within the NT. Work with designed peptides indicates that the PGAX motif is crucial for self-interactions between NT/CRD. Phosphorylation at position Ser6 (and Ser12) (a physiological modification) and the influence of ligand binding have minimal effect on this interaction. Lastly, galectin-3 molecules can interact weakly with each other via the F-faces of their CRDs, an interaction that appears to be assisted by their NTs. Overall, our results add insight to defining binding sites on galectin-3 beyond the canonical contact area for β-galactosides.
Original languageEnglish (US)
Pages (from-to)1-16
Number of pages16
JournalGlycobiology
DOIs
StatePublished - 2016

Keywords

  • adhesion
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  • Journal Article

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    Ippel, H., Miller, M. C., Vértesy, S., Zhang, Y., Cañada, F. J., Suylen, D., Umemoto, K., Romanò, C., Hackeng, T., & Tai, G. (2016). Intra- and inter-molecular interactions of human galectin-3 : assessment by full-assignment-based NMR. Glycobiology, 1-16. https://doi.org/10.1093/glycob/cww021