Abstract
In experimental diabetes, a number of intestinal brush-border hydrolases and transport systems are stimulated. In this study, we assessed possible effects of diabetes on the composition and membrane fluidity of rat intestinal brush-border membranes that might correlate with these functional changes. We found similar proportions of lipid and protein in the diabetic and control preparations, although there was a considerable increase in total membrane from the diabetic rats, presumably reflecting mucosal hyperplasia. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of membrane protein revealed an increase in the bands corresponding to sucrase-isomaltase, consistent with an increased enzyme activity of sucrase. Membrane lipid analysis revealed only a decrease in fatty acids of the neurtral lipid fraction of diabetics-a change that may well have occurred during membrane preparation. 1-6-Diphenyl-1,3,5-hexatriene fluorescence polarization data, obtained as a function of temperature, was similar for the diabetic and control rats, with a three-phase linear model superior to one- and two-phase linear or quadratic models. The overall composition of the intestinal brush-border membrane, unlike other plasma membranes, appears little affected by experimental diabetes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1053-1058 |
| Number of pages | 6 |
| Journal | Metabolism |
| Volume | 32 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 1983 |
Bibliographical note
Funding Information:From the Gastroenterology Research Laboratory, William S. Middleton Memorial Veterans Hospital. and the Departments of Pediatrics and Medicine, University of Wisconsin, Madison, Wis. Received for publication March 15, I983. Supported by grants AM 13927 and 5 F32HDO5873 from the National Institutes of Health and Veterans Administration Research funds. Dr Gourley is recipient of NIADDK Clinical Investigator Award #I KOB AMOlO??-01. Address reprint requests to Glenn R. Gourley, MD, Department of Pediatrics, University of Wisconsin Hospitals, 600 Highland Ave. Madison, WI 53792. 0 I983 by Grune & Stratton, Inc. 002&0495/83/321 J-0007$01.00/0
