Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism

Nathan E. Welty, Christopher Staley, Nico Ghilardi, Michael J. Sadowsky, Botond Z. Igyártó, Daniel H. Kaplan

Research output: Contribution to journalArticlepeer-review

117 Scopus citations

Abstract

Dendritic cells (DCs) in the intestinal lamina propria (LP) are composed of two CD103+ subsets that differ in CD11b expression. We report here that Langerin is expressed by human LP DCs and that transgenic human langerin drives expression in CD103+CD11b+ LP DCs in mice. This subset was ablated in huLangerin-DTA mice, resulting in reduced LP Th17 cells without affecting Th1 or T reg cells. Notably, cognate DC-T cell interactions were not required for Th17 development, as this response was intact in huLangerin-Cre I-Aβfl/fl mice. In contrast, responses to intestinal infection or flagellin administration were unaffected by the absence of CD103+CD11b+ DCs. huLangerin-DTA × BatF3-/- mice lacked both CD103+ LP DC subsets, resulting in defective gut homing and fewer LP T reg cells. Despite these defects in LP DCs and resident T cells, we did not observe alterations of intestinal microbial communities. Thus, CD103+ LP DC subsets control T cell homeostasis through both nonredundant and overlapping mechanisms.

Original languageEnglish (US)
Pages (from-to)2011-2024
Number of pages14
JournalJournal of Experimental Medicine
Volume210
Issue number10
DOIs
StatePublished - 2013

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