TY - JOUR
T1 - Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism
AU - Welty, Nathan E.
AU - Staley, Christopher
AU - Ghilardi, Nico
AU - Sadowsky, Michael J.
AU - Igyártó, Botond Z.
AU - Kaplan, Daniel H.
PY - 2013
Y1 - 2013
N2 - Dendritic cells (DCs) in the intestinal lamina propria (LP) are composed of two CD103+ subsets that differ in CD11b expression. We report here that Langerin is expressed by human LP DCs and that transgenic human langerin drives expression in CD103+CD11b+ LP DCs in mice. This subset was ablated in huLangerin-DTA mice, resulting in reduced LP Th17 cells without affecting Th1 or T reg cells. Notably, cognate DC-T cell interactions were not required for Th17 development, as this response was intact in huLangerin-Cre I-Aβfl/fl mice. In contrast, responses to intestinal infection or flagellin administration were unaffected by the absence of CD103+CD11b+ DCs. huLangerin-DTA × BatF3-/- mice lacked both CD103+ LP DC subsets, resulting in defective gut homing and fewer LP T reg cells. Despite these defects in LP DCs and resident T cells, we did not observe alterations of intestinal microbial communities. Thus, CD103+ LP DC subsets control T cell homeostasis through both nonredundant and overlapping mechanisms.
AB - Dendritic cells (DCs) in the intestinal lamina propria (LP) are composed of two CD103+ subsets that differ in CD11b expression. We report here that Langerin is expressed by human LP DCs and that transgenic human langerin drives expression in CD103+CD11b+ LP DCs in mice. This subset was ablated in huLangerin-DTA mice, resulting in reduced LP Th17 cells without affecting Th1 or T reg cells. Notably, cognate DC-T cell interactions were not required for Th17 development, as this response was intact in huLangerin-Cre I-Aβfl/fl mice. In contrast, responses to intestinal infection or flagellin administration were unaffected by the absence of CD103+CD11b+ DCs. huLangerin-DTA × BatF3-/- mice lacked both CD103+ LP DC subsets, resulting in defective gut homing and fewer LP T reg cells. Despite these defects in LP DCs and resident T cells, we did not observe alterations of intestinal microbial communities. Thus, CD103+ LP DC subsets control T cell homeostasis through both nonredundant and overlapping mechanisms.
UR - http://www.scopus.com/inward/record.url?scp=84885447087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885447087&partnerID=8YFLogxK
U2 - 10.1084/jem.20130728
DO - 10.1084/jem.20130728
M3 - Article
C2 - 24019552
AN - SCOPUS:84885447087
SN - 0022-1007
VL - 210
SP - 2011
EP - 2024
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 10
ER -