Intestinal CD8αα IELs derived from two distinct thymic precursors have staggered ontogeny

Roland Ruscher, S. Thera Lee, Oscar C. Salgado, Elise R. Breed, Sara H. Osum, Kristin A. Hogquist

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


CD8αα intraepithelial lymphocytes (IELs) are abundant T cells that protect the gut epithelium. Their thymic precursors (IELps) include PD-1+ type A and Tbet+ type B populations, which differ in their antigen-receptor specificities. To better understand CD8αα IEL ontogeny, we performed "time-stamp" fate mapping experiments and observed that it seeds the intestine predominantly during a narrow time window in early life. Adoptively transferred IELps parked better in the intestines of young mice than in adults. In young mice, both type A and type B IELps had an S1PR1+ and α4β7+ emigration- A nd mucosalhoming competent phenotype, while this was restricted to type A IELps in adults. Only CD8αα IELs established in early life were enriched in cells bearing type B IELp TCR usage. Together, our results suggest that the young intestine facilitates CD8αα IEL establishment and that early IELs are distinct from IELs established after this initial wave. These data provide novel insight into the ontogeny of CD8αα IELs.

Original languageEnglish (US)
Article number151959
JournalJournal of Experimental Medicine
Issue number8
StatePublished - Jul 2020

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health grants R37 AI39560 and PO1 AI35296 to K.A. Hogquist.

Publisher Copyright:
© 2020 Rockefeller University Press. All rights reserved.


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