Interspecies mitochondrial fusion between mouse and human mitochondria is rapid and efficient

Young Geol Yoon, Christopher L. Haug, Michael D Koob

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


A detailed molecular understanding of mitochondrial fusion and fission in mammalian cells is rapidly emerging. In this report, we demonstrate for the first time cross-species mitochondrial fusion between distantly related species using green and red fluorescent proteins targeted to the mitochondrial matrix. We found that mouse mitochondria were able to efficiently fuse to unmodified mitochondria of human cells and that the contents of the mitochondrial matrix were completely mixed in less than 4 h. We also observed that mitochondria from the mtDNA-less (ρ0) mouse cells can homogeneously fuse to the mitochondria of human cells. We were, however, unable to maintain human mitochondrial DNA in the mouse cells. These results indicate that mitochondrial fusion proteins in mouse and human cells have enough functional homology to mediate efficient cross-species mitochondrial fusion, but mouse nuclear and human mitochondrial genomes have not retained functional compatibility with one another.

Original languageEnglish (US)
Pages (from-to)223-229
Number of pages7
Issue number3
StatePublished - May 2007

Bibliographical note

Funding Information:
We would like to thank generous financial support from the Minnesota Medical Foundation, the Academic Health Center, and the Institute of Human Genetics of the University of Minnesota. This work was funded by National Institutes of Health (NINDS Grant No. NS052612).


  • Coevolution
  • Cybrid
  • Mitochondrial DNA
  • Mitochondrial fusion


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