Intermittent flooding of organic-rich soil promotes the formation of denitrification hot moments and hot spots

Abigail A. Tomasek, Miki Hondzo, Jessica Kozarek, Christopher M Staley, Ping Wang, Nicole Lurndahl, Michael J Sadowsky

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Anthropogenic activities have altered the nitrogen cycle, necessitating management on the landscape level. Isolated time periods and areas, termed hot moments and hot spots, respectively, frequently account for a large percentage of nitrate removal in aquatic ecosystems. A series of experiments were conducted to determine the effect of hydrologic connectivity on denitrification rates, gene abundances, and nitrous oxide fluxes. Experimental areas were divided into flooded (always inundated), floodzone (intermittently inundated), and nonfloodzone (not inundated) locations in low-organic and organic-rich soil. Our results demonstrate that intermittent flood events enhance denitrification rates from days to weeks after flooding, depending on the inundation period. Microbial analysis demonstrated that short-term flood events did not lead to increases in denitrifying gene abundances. Enhanced denitrification rates did not have a corresponding increase in the ratio of incomplete to complete denitrification. Incomplete to complete denitrification ratios were high in always-inundated low-organic sandy soil, peaking at 40%. Our results suggest that management strategies that promote hydrologic connectivity and intermittent flooding of organic-rich floodplain soils promote the formation of denitrification hot moments and hot spots, with relatively low incomplete denitrification (<3% of the total denitrification rates).

Original languageEnglish (US)
Article numbere02549
JournalEcosphere
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2019

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soil formation
denitrification
organic soil
flooding
connectivity
nitrogen cycle
alluvial soils
gene
nitrous oxide
organic soils
aquatic ecosystem
sandy soil
sandy soils
anthropogenic activities
floodplain
human activity
genes
nitrates
nitrate
rate

Keywords

  • denitrification
  • gene abundances
  • inundation
  • nitrous oxide
  • quantitative PCR
  • soil type

Cite this

Intermittent flooding of organic-rich soil promotes the formation of denitrification hot moments and hot spots. / Tomasek, Abigail A.; Hondzo, Miki; Kozarek, Jessica; Staley, Christopher M; Wang, Ping; Lurndahl, Nicole; Sadowsky, Michael J.

In: Ecosphere, Vol. 10, No. 1, e02549, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Staley, Christopher M

AU - Wang, Ping

AU - Lurndahl, Nicole

AU - Sadowsky, Michael J

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