Interleukin-1α regulates antimicrobial peptide expression in human keratinocytes

Mika Bando, Yuka Hiroshima, Masatoshi Kataoka, Yasuo Shinohara, Mark C. Herzberg, Karen F. Ross, Toshihiko Nagata, Jun Ichi Kido

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Human epidermis and epithelium serve as physiologic barriers to protect against noxious and infectious agents. Contributing to the defense against infection, epithelial cells express antimicrobial peptides (AMPs). The expression of AMPs in keratinocytes is generally regulated directly by bacteria and indirectly by proinflammatory cytokines. Bacteria may also regulate AMP expression by inducing keratinocyte expression of the autonomous proinflammatory cytokine, interleukin-1α (IL-1α). To test the hypothesis that AMP expression may be regulated by cell autonomous cytokines, we investigated the effect of IL-1α on the expression of AMPs in human keratinocytes (HaCaT cells) by microarray, northern blot, reverse transcriptase (RT)-PCR and western blot analyses. IL-1α increased expression of mRNA in a dose- and time-dependent manner specific for lipocalin 2, S100A8, S100A9 and secretory leukocyte protease inhibitor (SLPI) more than twofold relative to nonstimulated cells (control), and slightly upregulated S100A7 and β-defensin-2. Furthermore, the expression of lipocalin 2, S100A7, S100A8, S100A9 and SLPI proteins were upregulated by IL-1α. On the other hand, HaCaT cells expressed mRNA specific for other AMPs, including cystatin 3, adrenomedullin, RNase-7 and mucin 5, which were unaffected by IL-1α treatment. These results suggest that the autonomous keratinocyte cytokine, IL-1α, selectively upregulates the expression of AMPs which may modulate innate epithelial cell immunity in skin and mucosa.

Original languageEnglish (US)
Pages (from-to)532-537
Number of pages6
JournalImmunology and Cell Biology
Volume85
Issue number7
DOIs
StatePublished - Oct 1 2007

Fingerprint

Interleukin-1
Keratinocytes
Peptides
Secretory Leukocyte Peptidase Inhibitor
Cytokines
Epithelial Cells
Bacteria
Adrenomedullin
Defensins
Cystatin C
Messenger RNA
Mucins
Reverse Transcriptase Polymerase Chain Reaction
Epidermis
Northern Blotting
Immunity
Mucous Membrane
Up-Regulation
Epithelium
Western Blotting

Keywords

  • Antimicrobial peptides
  • IL-1α
  • Keratinocyte

Cite this

Interleukin-1α regulates antimicrobial peptide expression in human keratinocytes. / Bando, Mika; Hiroshima, Yuka; Kataoka, Masatoshi; Shinohara, Yasuo; Herzberg, Mark C.; Ross, Karen F.; Nagata, Toshihiko; Kido, Jun Ichi.

In: Immunology and Cell Biology, Vol. 85, No. 7, 01.10.2007, p. 532-537.

Research output: Contribution to journalArticle

Bando, Mika ; Hiroshima, Yuka ; Kataoka, Masatoshi ; Shinohara, Yasuo ; Herzberg, Mark C. ; Ross, Karen F. ; Nagata, Toshihiko ; Kido, Jun Ichi. / Interleukin-1α regulates antimicrobial peptide expression in human keratinocytes. In: Immunology and Cell Biology. 2007 ; Vol. 85, No. 7. pp. 532-537.
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