Interferon-α protects myeloma cell lines from dexamethasone-induced apoptosis

P. Liu, M. Oken, B. Van Ness

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Because of the increasing use of IFN-α in both induction and maintenance therapy for multiple myeloma (MM), its effect on growth and apoptosis of myeloma cells is important to consider. To investigate the role of IFN-α on the growth of myeloma cells, we have studied its effects on the response of interleukin 6 (IL-6)-dependent myeloma cell line (ANBL6) and IL-6-independent myeloma cell line (C2E3) in the presence of IL-6 and dexamethasone (Dex). We found that although IFN-α is a potent inhibitor of proliferation, it has only a minimal effect on induction of apoptosis. Moreover, we found IFN-α as well as IL-6 can significantly suppress dexamethasone-induced apoptosis. The suppression of apoptosis is concurrent with the induction of both AP-1 and STAT binding activity. We also found that IL-6 but not IFN-α up-regulates Bcl-X(L) expression. However, IL-6-mediated Bcl-X(L) expression is suppressed in the presence of Dex. Therefore, the expression of Bcl-X(L) does not account for the protection of Dex-induced apoptosis by IFN-α and IL-6. Taken together, our results suggest that IFN-α may counteract the beneficial effects of corticosteroids or perhaps other apoptosis inducing agents in the treatment of myeloma.

Original languageEnglish (US)
Pages (from-to)473-480
Number of pages8
JournalLeukemia
Volume13
Issue number3
DOIs
StatePublished - Jan 1 1999

Keywords

  • Dexamethasone
  • IL-6
  • Interferon-α
  • Myeloma

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