Interference with Fc signals increases an antibody response by T-cell-deprived cultures to a T-dependent antigen

Nicholas R St C Sinclair; Angela Panoskaltsis

doi:10.1016/0008-8749(87)90253-X

Interference with Fc signals increases an antibody response by T-cell-deprived cultures to a T-dependent antigen

Nicholas R St C Sinclair
, Angela Panoskaltsis

Pediatric Blood & Marrow Transplant & Cellular Therapy

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Affinity column purified goat anti-mouse immunoglobulin antibodies specific for the Fc portion of IgG increased an in vitro antibody response to a T-dependent antigen when T cells were limiting. Picogram amounts of specific anti-Fc antibody at culture initiation and nanogram quantities up to 3 days were required to demonstrate this effect. The demonstration of reconstitution by anti-Fc antibodies requires that the cultures be T-cell depleted and stimulated by antigen. These results support the concept that anti-Fc antibody and T cells block endogenously generated negative Fc signals.

Original language	English (US)
Pages (from-to)	465-470
Number of pages	6
Journal	Cellular Immunology
Volume	107
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(87)90253-X
State	Published - Jul 1987

Bibliographical note

Funding Information:
’ This work was supported by the Medical Research Council of Canada ’ To whom all correspondence should be addressed. 3 Abbreviations used: PFC, plaque-forming cells; Ig, immunoglobulin.

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title = "Interference with Fc signals increases an antibody response by T-cell-deprived cultures to a T-dependent antigen",

abstract = "Affinity column purified goat anti-mouse immunoglobulin antibodies specific for the Fc portion of IgG increased an in vitro antibody response to a T-dependent antigen when T cells were limiting. Picogram amounts of specific anti-Fc antibody at culture initiation and nanogram quantities up to 3 days were required to demonstrate this effect. The demonstration of reconstitution by anti-Fc antibodies requires that the cultures be T-cell depleted and stimulated by antigen. These results support the concept that anti-Fc antibody and T cells block endogenously generated negative Fc signals.",

author = "Sinclair, \{Nicholas R St C\} and Angela Panoskaltsis",

note = "Funding Information: {\textquoteright} This work was supported by the Medical Research Council of Canada {\textquoteright} To whom all correspondence should be addressed. 3 Abbreviations used: PFC, plaque-forming cells; Ig, immunoglobulin.",

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AU - Sinclair, Nicholas R St C

AU - Panoskaltsis, Angela

N1 - Funding Information: ’ This work was supported by the Medical Research Council of Canada ’ To whom all correspondence should be addressed. 3 Abbreviations used: PFC, plaque-forming cells; Ig, immunoglobulin.

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N2 - Affinity column purified goat anti-mouse immunoglobulin antibodies specific for the Fc portion of IgG increased an in vitro antibody response to a T-dependent antigen when T cells were limiting. Picogram amounts of specific anti-Fc antibody at culture initiation and nanogram quantities up to 3 days were required to demonstrate this effect. The demonstration of reconstitution by anti-Fc antibodies requires that the cultures be T-cell depleted and stimulated by antigen. These results support the concept that anti-Fc antibody and T cells block endogenously generated negative Fc signals.

AB - Affinity column purified goat anti-mouse immunoglobulin antibodies specific for the Fc portion of IgG increased an in vitro antibody response to a T-dependent antigen when T cells were limiting. Picogram amounts of specific anti-Fc antibody at culture initiation and nanogram quantities up to 3 days were required to demonstrate this effect. The demonstration of reconstitution by anti-Fc antibodies requires that the cultures be T-cell depleted and stimulated by antigen. These results support the concept that anti-Fc antibody and T cells block endogenously generated negative Fc signals.

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UR - https://www.scopus.com/pages/publications/0023260070#tab=citedBy

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ER -

Interference with Fc signals increases an antibody response by T-cell-deprived cultures to a T-dependent antigen

Abstract

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