Abstract
Affinity column purified goat anti-mouse immunoglobulin antibodies specific for the Fc portion of IgG increased an in vitro antibody response to a T-dependent antigen when T cells were limiting. Picogram amounts of specific anti-Fc antibody at culture initiation and nanogram quantities up to 3 days were required to demonstrate this effect. The demonstration of reconstitution by anti-Fc antibodies requires that the cultures be T-cell depleted and stimulated by antigen. These results support the concept that anti-Fc antibody and T cells block endogenously generated negative Fc signals.
Original language | English (US) |
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Pages (from-to) | 465-470 |
Number of pages | 6 |
Journal | Cellular Immunology |
Volume | 107 |
Issue number | 2 |
DOIs | |
State | Published - Jul 1987 |
Bibliographical note
Funding Information:’ This work was supported by the Medical Research Council of Canada ’ To whom all correspondence should be addressed. 3 Abbreviations used: PFC, plaque-forming cells; Ig, immunoglobulin.