Taurine/α-ketoglutarate (αKG) dioxygenase, or TauD, is a mono-nuclear non-heme iron hydroxylase that couples the oxidative decarboxylation of αKG to the decomposition of taurine, forming sulfite and aminoacetaldehyde. Prior studies revealed that taurine-free TauD catalyzes an O2- and αKG-dependent self-hydroxylation reaction involving Tyr-73, yielding an Fe(III)-catecholate chromophore with a λmax of 550 nm. Here, a chromophore (λmax 720 nm) is described and shown to arise from O2-dependent self-hydroxylation of TauD in the absence of αKG, but requiring the product succinate. A similar chromophore rapidly develops with the alternative oxidant H2O2. Resonance Raman spectra indicate that the ≈700-nm chromophore also arises from an Fe(III)-catecholate species, and site-directed mutagenesis studies again demonstrate Tyr-73 involvement. The ≈700-nm and 550-nm species are shown to interconvert by the addition or removal of bicarbonate, consistent with the αKG-derived CO2 remaining tightly bound to the oxidized metal site as bicarbonate. The relevance of the metal-bound bicarbonate in TauD to reactions of other members of this enzyme family is discussed.
|Original language||English (US)|
|Number of pages||6|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - Apr 1 2003|